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Durable mesenchymal stem cell labelling by using polyhedral superparamagnetic iron oxide nanoparticles.
- Source :
-
Chemistry (Weinheim an der Bergstrasse, Germany) [Chemistry] 2009 Nov 16; Vol. 15 (45), pp. 12417-25. - Publication Year :
- 2009
-
Abstract
- Small polyhedral superparamagnetic iron oxide (SPIO) nanoparticles (<10 nm) coated with a thin layer of silica were prepared (SPIO@SiO(2) and SPIO@SiO(2)-NH(2)). Surface modification of the small polyhedral silica-coated SPIO nanoparticles with amines led to substantially higher mesenchymal stem cell (MSC) labelling efficiency without the use of additional transfecting agents. Therefore, amine surface-modified nanoparticles (SPIO@ SiO(2)-NH(2)) appeared to be the preferred candidate for MSC labelling. In vitro studies demonstrated that controlled labelling of SPIO@SiO(2) and SPIO@SiO(2)-NH(2) did not cause MSC death or proliferation inhibition. MSCs labelled with SPIO@SiO(2)-NH(2) nanoparticles retained differentiation potential and showed osteogenic, adipogenic and chondrogenic differentiations. The noncytotoxic polyhedral SPIO@SiO(2)-NH(2) nanoparticle-labelled MSCs were successfully implanted in rabbit brain and erector spinae muscle, and demonstrated long-lasting, durable MRI labelling efficacy after 8-12 weeks.
- Subjects :
- Animals
Contrast Media pharmacokinetics
Magnetic Resonance Imaging methods
Nanoparticles
Rabbits
Receptors, Transferrin metabolism
Amines chemistry
Contrast Media chemistry
Ferrosoferric Oxide chemistry
Iron pharmacokinetics
Mesenchymal Stem Cells cytology
Receptors, Transferrin chemistry
Silicon Dioxide chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1521-3765
- Volume :
- 15
- Issue :
- 45
- Database :
- MEDLINE
- Journal :
- Chemistry (Weinheim an der Bergstrasse, Germany)
- Publication Type :
- Academic Journal
- Accession number :
- 19834937
- Full Text :
- https://doi.org/10.1002/chem.200901548