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GC-MS analysis of the antioxidant active fractions of Micromeria juliana with anticholinesterase activity.
- Source :
-
Natural product communications [Nat Prod Commun] 2009 Sep; Vol. 4 (9), pp. 1271-6. - Publication Year :
- 2009
-
Abstract
- The aerial parts of Micromeria juliana (L.) Bentham ex Reichb. were extracted with light petroleum, acetone and methanol, successively. The antioxidant activity of different concentrations of the extracts was evaluated using different antioxidant tests, namely total antioxidant (lipid peroxidation inhibition activity), DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, ferric reducing power, and metal chelating. Total antioxidant activity was determined using the beta-carotene-linoleic acid assay. Unexpectedly, the light petroleum extract exhibited strong lipid peroxidation inhibition activity. The extract was fractionated on a silica gel column and the antioxidant activity of the fractions was determined by the beta-carotene-linoleic assay at 25 microg/mL concentration. The fractions that exhibited more than 50% inhibition activity were analysed by GC and GC/MS; thus, the structure of fourteen compounds were elucidated. In addition, acetyl- and butyryl-cholinesterase inhibitory activities of the extracts were also determined in vitro. The light petroleum and acetone extracts were found to have mild butyrylcholinesterase inhibitory activity.
- Subjects :
- Acetylcholinesterase metabolism
Antioxidants chemistry
Antioxidants pharmacology
Biphenyl Compounds metabolism
Chelating Agents chemistry
Chelating Agents isolation & purification
Chelating Agents pharmacology
Cholinesterase Inhibitors chemistry
Cholinesterase Inhibitors pharmacology
Ferrous Compounds metabolism
Gas Chromatography-Mass Spectrometry
Picrates metabolism
Plant Components, Aerial chemistry
Plant Extracts chemistry
beta Carotene metabolism
Antioxidants isolation & purification
Cholinesterase Inhibitors isolation & purification
Lamiaceae chemistry
Plant Extracts pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1934-578X
- Volume :
- 4
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Natural product communications
- Publication Type :
- Academic Journal
- Accession number :
- 19831043