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Measurement of DPOAE after ischemia/reperfusion injury of the cochlea in gerbils.

Measurement of DPOAE after ischemia/reperfusion injury of the cochlea in gerbils.

Authors :
Watanabe F
Hakuba N
Gyo K
Source :
Neuroscience letters [Neurosci Lett] 2009 Dec 25; Vol. 467 (2), pp. 135-8. Date of Electronic Publication: 2009 Oct 12.
Publication Year :
2009

Abstract

The effects on distortion product otoacoustic emissions (DPOAEs) during the late phase of ischemia/reperfusion injury in the cochlea were studied. Ischemia/reperfusion injury was induced in a gerbil model by occluding both vertebral arteries for 15min. Hearing was assessed by recording compound action potentials (CAPs) before, during, and 7 days after ischemia. The histological changes in the hair cells were evaluated in specimens stained with rhodamine-phalloidin and Hoechst 33342. The average increase in CAP threshold 7 days after ischemia was 16.3+/-8.0dB at 8kHz. In contrast, interruption of the blood supply to the cochlea decreased the DPOAE amplitudes to the noise floor; this usually recovered to the same level as that seen under pre-ischemic conditions 7 days after ischemia. Histologically, the mean respective losses of inner and outer hair cells (IHCs and OHCs, respectively) of the inner ear were 26.5% and 3.3% in the basal turn, respectively. These results indicate that in gerbils OHCs are tolerant to ischemia/reperfusion injury pathologically and physiologically because DPOAE is closely related to the active process of OHCs and is a useful test to examine OHC function.

Details

Language :
English
ISSN :
1872-7972
Volume :
467
Issue :
2
Database :
MEDLINE
Journal :
Neuroscience letters
Publication Type :
Academic Journal
Accession number :
19822188
Full Text :
https://doi.org/10.1016/j.neulet.2009.10.023