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Differential immunoproteomics enables identification of Metarhizium anisopliae proteins related to Rhipicephalus microplus infection.
- Source :
-
Research in microbiology [Res Microbiol] 2009 Dec; Vol. 160 (10), pp. 824-8. Date of Electronic Publication: 2009 Oct 02. - Publication Year :
- 2009
-
Abstract
- Differential immunoproteomics was applied to identify proteins secreted by Metarhizium anisopliae induced by the Rhipicephalus microplus cuticle. In addition, IgG anti-spore surface proteins were used for searching for proteins possibly involved in early stages of fungus versus tick infection. LC-MS/MS of differentially secreted proteins led to the identification of proteases (carboxypeptidase and Pr1A), chitinase, carboxylic acid transport and proline-rich protein. Differential immunoproteomics strategy facilitated the detection and the identification of new proteins related to M. anisopliae host-pathogen interaction and could be used in further works to identify novel proteins related to other microbial infection systems.
- Subjects :
- Animals
Fungal Proteins immunology
Fungal Proteins metabolism
Host-Pathogen Interactions genetics
Immunoblotting
Metarhizium isolation & purification
Metarhizium metabolism
Proteomics methods
Rhipicephalus metabolism
Rhipicephalus microbiology
Tick Infestations microbiology
Fungal Proteins analysis
Metarhizium chemistry
Proteome metabolism
Rhipicephalus pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 1769-7123
- Volume :
- 160
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Research in microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 19800970
- Full Text :
- https://doi.org/10.1016/j.resmic.2009.09.012