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Pathogenic bacteria and dead cells are internalized by a unique subset of Peyer's patch dendritic cells that express lysozyme.
- Source :
-
Gastroenterology [Gastroenterology] 2010 Jan; Vol. 138 (1), pp. 173-84.e1-3. Date of Electronic Publication: 2009 Oct 01. - Publication Year :
- 2010
-
Abstract
- Background & Aims: Lysozyme has an important role in preventing bacterial infection. In the gastrointestinal tract, lysozyme is thought to be mainly expressed by Paneth cells of the crypt epithelium. We investigated its expression in the Peyer's patch, a major intestinal site of antigen sampling and pathogen entry.<br />Methods: We performed immunostaining on normal and Salmonella Typhimurium-infected intestinal samples and analyzed them by confocal microscopy and flow cytometry.<br />Results: In Peyer's patch of mouse, rat, and human, lysozyme was strongly expressed in the germinal center of follicles by tingible body macrophages and in the subepithelial dome by a subset of myeloid dendritic cells (DC). Among DC subsets from mouse Peyer's patches, these lysozyme-expressing DC displayed the highest surface expression of class II major histocompatibility complex and costimulatory molecules; they were the most efficient at capturing microspheres in vitro. Moreover, they were the main DC subset involved in bacterial pathogen uptake and in dead cell clearance, including M cells.<br />Conclusions: The subepithelial dome of Peyer's patches contains a unique population of intestinal DC that secretes high levels of lysozyme and internalizes bacteria and dead cells.<br /> (Copyright 2010 AGA Institute. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Antigens, Differentiation metabolism
CD11b Antigen metabolism
CD11c Antigen metabolism
CX3C Chemokine Receptor 1
Enteritis immunology
Enteritis metabolism
Enteritis microbiology
Female
Flow Cytometry
Humans
Macrophages enzymology
Macrophages immunology
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Microscopy, Confocal
Myeloid Cells enzymology
Myeloid Cells immunology
Myeloid Cells microbiology
Rats
Receptors, Chemokine metabolism
Dendritic Cells enzymology
Dendritic Cells immunology
Dendritic Cells microbiology
Muramidase metabolism
Peyer's Patches cytology
Peyer's Patches enzymology
Peyer's Patches immunology
Salmonella Infections immunology
Salmonella Infections metabolism
Salmonella typhimurium pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 1528-0012
- Volume :
- 138
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 19800337
- Full Text :
- https://doi.org/10.1053/j.gastro.2009.09.051