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Chromatin flow cytometry identifies changes in epigenetic cell states.
- Source :
-
Cells, tissues, organs [Cells Tissues Organs] 2010; Vol. 191 (3), pp. 167-74. Date of Electronic Publication: 2009 Sep 18. - Publication Year :
- 2010
-
Abstract
- We report flow cytometric protocols that quantitatively display the levels of different histone modifications on the single cell level. This method allows the rapid and simultaneous analysis of the global levels of chromatin marks in combination with other flow cytometric features. We show that chromatin flow cytometry identifies changes in epigenetic marks, such as histone acetylation and methylation, in drug-treated and differentiated mouse embryonic stem cell populations.<br /> (2009 S. Karger AG, Basel.)
- Subjects :
- Acetylation drug effects
Animals
Azepines pharmacology
Cell Differentiation
Cells, Cultured
Chromatin metabolism
Embryonic Stem Cells cytology
Embryonic Stem Cells drug effects
Histone Deacetylase Inhibitors pharmacology
Histone-Lysine N-Methyltransferase antagonists & inhibitors
Histones metabolism
Hydroxamic Acids pharmacology
Methylation drug effects
Mice
Quinazolines pharmacology
Chromatin genetics
Embryonic Stem Cells physiology
Epigenesis, Genetic
Flow Cytometry methods
Subjects
Details
- Language :
- English
- ISSN :
- 1422-6421
- Volume :
- 191
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Cells, tissues, organs
- Publication Type :
- Academic Journal
- Accession number :
- 19776550
- Full Text :
- https://doi.org/10.1159/000240247