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Differences in cytotoxicity versus pro-inflammatory potency of different PM fractions in human epithelial lung cells.
- Source :
-
Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2010 Feb; Vol. 24 (1), pp. 29-39. Date of Electronic Publication: 2009 Sep 20. - Publication Year :
- 2010
-
Abstract
- Air pollution in Milan causes health concern due to the high concentrations of particulate matter (PM10 and PM2.5). The aim of this study was to investigate possible seasonal differences in PM10 and PM2.5 chemical composition and their biological effects on pro-inflammatory cytokine release and cytotoxicity. The PM was sampled during winter and summer seasons. The winter PMs had higher levels of PAHs than the summer samples which contained a greater amount of mineral dust elements. The PM toxicity was tested in the human pulmonary epithelial cell lines BEAS-2B and A549. The winter PMs were more cytotoxic than summer samples, whereas the summer PM10 exhibited a higher pro-inflammatory potential, as measured by ELISA. This inflammatory potential seemed partly due to biological components such as bacterial lipopolysaccharides (LPS), as evaluated by the use of Polymixin B. Interestingly, in the BEAS-2B cells the winter PM2.5 reduced proliferation due to a mitotic delay/arrest, while no such effects were observed in the A549 cells. These results underline that the in vitro responsiveness to PM may be cell line dependent and suggest that the PM different properties may trigger different endpoints such as inflammation, perturbation of cell cycle and cell death.
- Subjects :
- Carbon analysis
Cell Line
Chromatography, High Pressure Liquid
Elements
Humans
Indicators and Reagents
Inflammation pathology
Interleukin-8 biosynthesis
Italy
Lung drug effects
Particle Size
Particulate Matter analysis
Polycyclic Aromatic Hydrocarbons analysis
Pulmonary Alveoli cytology
Pulmonary Alveoli drug effects
Seasons
Cell Survival drug effects
Epithelial Cells drug effects
Inflammation chemically induced
Lung cytology
Particulate Matter toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3177
- Volume :
- 24
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Toxicology in vitro : an international journal published in association with BIBRA
- Publication Type :
- Academic Journal
- Accession number :
- 19772914
- Full Text :
- https://doi.org/10.1016/j.tiv.2009.09.013