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Signal requirements for activation of leukaemic T cells from a chronic lymphocytic leukaemia (T-CLL).
- Source :
-
Clinical and experimental immunology [Clin Exp Immunol] 1990 Oct; Vol. 82 (1), pp. 108-13. - Publication Year :
- 1990
-
Abstract
- In order to define the signal requirements for leukaemic T cell activation, the proliferation and interleukin-2 (IL-2) production of peripheral lymphocytes from a patient with a HTLV-I-, CD4+, CD45RA+ CD45RO+ CD25- T-CLL were evaluated after the delivery of different stimuli. Unlike resting CD4+ normal T lymphocytes that can be activated only by a two-signal stimulation, T-CLL cells proliferated and released IL-2 in response to a pair of anti-CD2 monoclonal antibodies (MoAbs) or concanavalin A (Con A) in the absence of both accessory cells (AC) and phorbol myristate acetate (PMA). The two stimuli were also able to induce CD25 expression within 12-20 h on the majority of T-CLL cells. A response to anti-CD3 and anti-CD28 MoAbs was detected only in the presence of PMA, similar to that observed in normal resting T lymphocytes matched for phenotype. Both Con A- and CD2-induced proliferation were strongly inhibited by the addition of anti-CD25 MoAb. Furthermore, T-CLL lymphocytes acquired anti-tumour lytic activity after culture in the presence of PMA and ionomycin. We conclude that HTLV1- CD25- T-CLL can be characterized not only by morphological and phenotypical studies but also on the basis of signal requirements for cell activation.
- Subjects :
- Antibodies, Monoclonal immunology
Antigens, Differentiation, T-Lymphocyte immunology
CD2 Antigens
CD28 Antigens
CD3 Complex
Concanavalin A
Cytotoxicity, Immunologic immunology
Flow Cytometry
Humans
Interleukin-2 biosynthesis
Male
Middle Aged
Receptors, Antigen, T-Cell immunology
Receptors, Immunologic immunology
Receptors, Interleukin-2 biosynthesis
Signal Transduction
Tetradecanoylphorbol Acetate
Leukemia, Lymphocytic, Chronic, B-Cell immunology
Lymphocyte Activation immunology
T-Lymphocytes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0009-9104
- Volume :
- 82
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Clinical and experimental immunology
- Publication Type :
- Academic Journal
- Accession number :
- 1976463
- Full Text :
- https://doi.org/10.1111/j.1365-2249.1990.tb05412.x