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[HPLC fingerprint of flavonoids and phenols of Dendrobium nobile].

Authors :
Ou HJ
Cheng JL
Li XX
Zhan RT
Liang YZ
Xu JY
Xu H
Yan P
Source :
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials [Zhong Yao Cai] 2009 Jun; Vol. 32 (6), pp. 871-4.
Publication Year :
2009

Abstract

Objective: To establish HPLC fingerprint of flavonoids and phenols of Dendrobium nobile.<br />Methods: Phenomenex prodigy ODS(3) C18 column (250 mm x 4.6 mm, 5 microm) was used with a mixture of acetonitrile-0.1% acetic acid as the mobile phase in a gradient mode, the column temperature was 25 degrees C, the flow rate was 1.0 mL/min, and the detection wavelength was 254 nm.<br />Results: The flavonoids and phenols of Dendrobium nobile were well separated, and 10 fingerprint peaks in common were confirmed.<br />Conclusion: This method is simple, accurate with good reproducibility, and can be used specifically for the quality control of Dendrobium nobile.

Details

Language :
Chinese
ISSN :
1001-4454
Volume :
32
Issue :
6
Database :
MEDLINE
Journal :
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
Publication Type :
Academic Journal
Accession number :
19764325