Back to Search
Start Over
NDRG1 and CRK-I/II are regulators of endothelial cell migration under Intermittent Hypoxia.
- Source :
-
Angiogenesis [Angiogenesis] 2009; Vol. 12 (4), pp. 339-54. Date of Electronic Publication: 2009 Sep 17. - Publication Year :
- 2009
-
Abstract
- Intermittent Hypoxia (IH) that develops in neovascularized solid tumours has been described to positively influence the tumour growth by modulating the behaviour of cancer cells as well as of endothelial cells. However, the molecular mechanisms regulated by IH still remain poorly understood. In this work, the effects of IH were investigated on endothelial cells by a proteomic approach. Protein abundance variations were studied using fluorescent 2D-Differential in Gel Electrophoresis (2D-DIGE). Amongst the proteins of which the abundance varied under IH, NDRG1 and CRK-I/II were identified by mass spectrometry. These proteins have already been described to influence cancer cell migration as well as the angiogenic processes in solid tumours. Since an increase in endothelial cell migration under IH was evidenced in our previous work, the involvement of NDRG1 and CRK-I/II proteins in endothelial cell migration under IH was determined by silencing the expression of both proteins using siRNA. The results revealed that NDRG1 and CRK-I/II are indeed regulators of endothelial cell migration under intermittent hypoxia: silencing of CRK-I/II resulted in an increase in endothelial cell migration, whereas the invalidation of NDRG1 decreased it. These results give news insight regarding the effects of IH on endothelial cell migration and hence on neoangiogenesis.
- Subjects :
- Cell Cycle Proteins antagonists & inhibitors
Cell Cycle Proteins genetics
Cell Line, Transformed drug effects
Cell Line, Transformed metabolism
Cell Movement
Drug Administration Schedule
Electrophoresis, Gel, Two-Dimensional
Endothelial Cells drug effects
Endothelial Cells metabolism
Gene Expression Profiling
Humans
Hybrid Cells cytology
Hybrid Cells drug effects
Hybrid Cells metabolism
Image Processing, Computer-Assisted
Intracellular Signaling Peptides and Proteins antagonists & inhibitors
Intracellular Signaling Peptides and Proteins genetics
Mass Spectrometry
Oxygen administration & dosage
Oxygen pharmacology
Polymerase Chain Reaction methods
Proteomics
Proto-Oncogene Proteins c-crk antagonists & inhibitors
Proto-Oncogene Proteins c-crk genetics
RNA Interference
RNA, Small Interfering pharmacology
Cell Cycle Proteins physiology
Cell Hypoxia physiology
Endothelial Cells cytology
Intracellular Signaling Peptides and Proteins physiology
Proto-Oncogene Proteins c-crk physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1573-7209
- Volume :
- 12
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Angiogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 19760510
- Full Text :
- https://doi.org/10.1007/s10456-009-9156-2