Back to Search
Start Over
Curcumin reduces oxidative and nitrative DNA damage through balancing of oxidant-antioxidant status in hamsters infected with Opisthorchis viverrini.
- Source :
-
Molecular nutrition & food research [Mol Nutr Food Res] 2009 Oct; Vol. 53 (10), pp. 1316-28. - Publication Year :
- 2009
-
Abstract
- Opisthorchis viverrini (OV) infection is endemic in northeastern Thailand. We have previously reported that OV infection induces oxidative and nitrative DNA damage via chronic inflammation, which contributes to the disease and cholangiocarcinogenesis. Here, we examined the effect of curcumin, an antioxidant, on pathogenesis in OV-infected hamsters. DNA lesions were detected by double immunofluorescence and the hepatic expression of oxidant-generating and antioxidant genes was assessed by quantitative RT-PCR analysis. Dietary 1.0% curcumin significantly decreased OV-induced accumulation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), an oxidative DNA lesion, and 8-nitroguanine, a nitrative DNA lesion, in the nucleus of bile duct epithelial and inflammatory cells. Expression of oxidant-generating genes (inducible nitric oxide synthase; iNOS, its nuclear transcriptional factor, NF-kappaB, and cyclooxygenase-2), and plasma levels of nitrate, malondialdehyde, and alanine aminotransferase, were also decreased in curcumin-treated group. In contrast, curcumin increased the mRNA expression of antioxidant enzymes (Mn-superoxide dismutase and catalase), and ferric-reducing anti-oxidant power in the plasma. In conclusion, curcumin reduced oxidative and nitrative DNA damage by suppression of oxidant-generating genes and enhancement of antioxidant genes, leading to inhibition of oxidative and nitrative stress. Therefore, curcumin may be used as a chemopreventive agent to reduce the severity of OV-associated diseases and the risk of cholangiocarcinoma (CCA).
- Subjects :
- Animals
Catalase genetics
Catalase metabolism
Cricetinae
Cyclooxygenase 2 genetics
Cyclooxygenase 2 metabolism
Dose-Response Relationship, Drug
Gene Expression drug effects
Liver drug effects
Liver enzymology
Liver pathology
Malondialdehyde blood
Mesocricetus
NF-kappa B genetics
NF-kappa B metabolism
Nitrates blood
Nitric Oxide Synthase Type II genetics
Nitric Oxide Synthase Type II metabolism
Opisthorchiasis metabolism
Opisthorchiasis parasitology
Opisthorchis drug effects
Opisthorchis physiology
Parasite Egg Count
Proliferating Cell Nuclear Antigen metabolism
Superoxide Dismutase genetics
Superoxide Dismutase metabolism
Antimutagenic Agents pharmacology
Curcumin pharmacology
DNA Damage drug effects
Opisthorchiasis pathology
Oxidative Stress drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1613-4133
- Volume :
- 53
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Molecular nutrition & food research
- Publication Type :
- Academic Journal
- Accession number :
- 19753608
- Full Text :
- https://doi.org/10.1002/mnfr.200800567