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PCR detection of ranavirus in adult anurans from the Louisville Zoological Garden.
- Source :
-
Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians [J Zoo Wildl Med] 2009 Sep; Vol. 40 (3), pp. 559-63. - Publication Year :
- 2009
-
Abstract
- Ranaviruses are known to cause mortality in a variety of anuran species and have the potential to significantly impact wild and captive frog populations. In this study, 16 captive frogs and toads from the Louisville Zoological Garden were examined for the presence of ranavirus; this group included 14 Cope's grey tree frogs (Hyla chrysoscelis), an American toad (Bufo americanus), and a southern toad (Bufo terrestris). All animals were wild caught and were evaluated via polymerase chain reaction (PCR), while animals that died were also assessed via histologic study to understand the role of ranaviral disease in these specimens. Of the animals that died, 82% were positive for ranavirus via PCR. Multiple swab samples collected over time from live tree frogs were positive for ranavirus via PCR. These findings reveal that ranaviral infection in captive adult anurans may occur without clinical signs or consistent histopathologic lesions.
- Subjects :
- Animals
Animals, Zoo virology
DNA Virus Infections diagnosis
DNA Virus Infections epidemiology
DNA Virus Infections transmission
Disease Outbreaks veterinary
Fatal Outcome
Ranavirus genetics
Anura virology
DNA Virus Infections veterinary
Polymerase Chain Reaction veterinary
Ranavirus isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1042-7260
- Volume :
- 40
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians
- Publication Type :
- Academic Journal
- Accession number :
- 19746873
- Full Text :
- https://doi.org/10.1638/2008-0076.1