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Extended-spectrum beta-lactamases among Enterobacteriaceae isolated in a public hospital in Brazil.
- Source :
-
Revista do Instituto de Medicina Tropical de Sao Paulo [Rev Inst Med Trop Sao Paulo] 2009 Jul-Aug; Vol. 51 (4), pp. 203-9. - Publication Year :
- 2009
-
Abstract
- Extended-spectrum beta-lactamases (ESBL) in enterobacteria are recognized worldwide as a great hospital problem. In this study, 127 ESBL-producing Enterobacteriaceae isolated in one year from inpatients and outpatients at a public teaching hospital at São Paulo, Brazil, were submitted to analysis by PCR with specific primers for bla SHV, bla TEM and bla CTX-M genes. From the 127 isolates, 96 (75.6%) Klebsiella pneumoniae, 12 (9.3%) Escherichia coli, 8 (6.2%) Morganella morganii, 3 (2.3%) Proteus mirabilis, 2 (1.6%) Klebsiella oxytoca, 2 (1.6%) Providencia rettgeri, 2 (1.6%) Providencia stuartti, 1 (0.8%) Enterobacter aerogenes and 1 (0.8%) Enterobacter cloacae were identified as ESBL producers. Bla SHV, bla TEM and bla CTX-M were detected in 63%, 17.3% and 33.9% strains, respectively. Pulsed field gel eletrophoresis genotyping of K. pneumoniae revealed four main molecular patterns and 29 unrelated profiles. PCR results showed a high variety of ESBL groups among strains, in nine different species. The results suggest the spread of resistance genes among genetically different strains of ESBL-producing K. pneumoniae in some hospital wards, and also that some strongly related strains were identified in different hospital wards, suggesting clonal spread in the institutional environment.
- Subjects :
- Brazil
Drug Resistance, Multiple, Bacterial genetics
Electrophoresis, Gel, Pulsed-Field
Enterobacteriaceae drug effects
Enterobacteriaceae Infections prevention & control
Escherichia coli isolation & purification
Genotype
Hospitals, Teaching
Humans
Klebsiella pneumoniae genetics
Polymerase Chain Reaction
beta-Lactamases genetics
Enterobacteriaceae genetics
Enterobacteriaceae Infections microbiology
beta-Lactamases biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1678-9946
- Volume :
- 51
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Revista do Instituto de Medicina Tropical de Sao Paulo
- Publication Type :
- Academic Journal
- Accession number :
- 19739000
- Full Text :
- https://doi.org/10.1590/s0036-46652009000400005