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Isolation of fetal trophoblast cells from peripheral blood of pregnant women.

Authors :
Mueller UW
Hawes CS
Wright AE
Petropoulos A
DeBoni E
Firgaira FA
Morley AA
Turner DR
Jones WR
Source :
Lancet (London, England) [Lancet] 1990 Jul 28; Vol. 336 (8709), pp. 197-200.
Publication Year :
1990

Abstract

Fetal trophoblast cells were isolated from maternal peripheral blood by means of murine monoclonal antibodies of high specificity and affinity for human syncytiotrophoblast and nonvillous cytotrophoblast cells. The cells were isolated in sufficient numbers to allow polymerase chain reaction (PCR) amplification of the Y-chromosome-specific DNA sequence from the peripheral blood of thirteen pregnant women. The fetal sex predicted by PCR analysis of the isolated trophoblast cells accorded with that ascertained by karyotyping of chorionic villus samples in eleven of twelve women studied in early pregnancy and with the sex of the baby on delivery in one woman studied at 34 weeks' gestation. Isolation of these fetal cells could allow noninvasive diagnosis of a wide range of inherited disorders.

Details

Language :
English
ISSN :
0140-6736
Volume :
336
Issue :
8709
Database :
MEDLINE
Journal :
Lancet (London, England)
Publication Type :
Academic Journal
Accession number :
1973769
Full Text :
https://doi.org/10.1016/0140-6736(90)91731-o