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Isolation of fetal trophoblast cells from peripheral blood of pregnant women.
- Source :
-
Lancet (London, England) [Lancet] 1990 Jul 28; Vol. 336 (8709), pp. 197-200. - Publication Year :
- 1990
-
Abstract
- Fetal trophoblast cells were isolated from maternal peripheral blood by means of murine monoclonal antibodies of high specificity and affinity for human syncytiotrophoblast and nonvillous cytotrophoblast cells. The cells were isolated in sufficient numbers to allow polymerase chain reaction (PCR) amplification of the Y-chromosome-specific DNA sequence from the peripheral blood of thirteen pregnant women. The fetal sex predicted by PCR analysis of the isolated trophoblast cells accorded with that ascertained by karyotyping of chorionic villus samples in eleven of twelve women studied in early pregnancy and with the sex of the baby on delivery in one woman studied at 34 weeks' gestation. Isolation of these fetal cells could allow noninvasive diagnosis of a wide range of inherited disorders.
- Subjects :
- Cell Separation
Chorionic Villi Sampling
Evaluation Studies as Topic
False Positive Reactions
Female
Genetic Diseases, Inborn diagnosis
Humans
Infant, Newborn
Karyotyping
Male
Oligonucleotide Probes
Polymerase Chain Reaction
Pregnancy genetics
Pregnancy Trimester, First
Pregnancy Trimester, Third
Sex Determination Analysis
Antibodies, Monoclonal
DNA analysis
Pregnancy blood
Trophoblasts cytology
Y Chromosome analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0140-6736
- Volume :
- 336
- Issue :
- 8709
- Database :
- MEDLINE
- Journal :
- Lancet (London, England)
- Publication Type :
- Academic Journal
- Accession number :
- 1973769
- Full Text :
- https://doi.org/10.1016/0140-6736(90)91731-o