Back to Search
Start Over
Cobalt toxicity: chemical and radiological combined effects on HaCaT keratinocyte cell line.
- Source :
-
Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2010 Feb; Vol. 24 (1), pp. 92-8. Date of Electronic Publication: 2009 Sep 06. - Publication Year :
- 2010
-
Abstract
- Cobalt (Co) is an essential trace element well known as a constituent of vitamin B(12), but different compounds of Co are also described as highly toxic and/or radiotoxic for individuals or the environment. In nuclear power plants, (58)Co and (60)Co are radioactive isotopes of cobalt present as activation products of stable Co and Ni used in alloys. Skin exposure is a current occupational risk in the hard metal and nuclear industries. As biochemical and molecular cobalt-induced toxicological mechanisms are not fully identified, we investigated cobalt toxicity in a model human keratinocyte cell line, HaCaT. In this study, we propose a model to determine the in vitro chemical impact on cell viability of a soluble form of cobalt (CoCl(2)) with or without gamma-ray doses to mimic contamination by (60)Co, to elucidate the mechanisms of cobalt intracellular chemical and radiological toxicity. Intracellular cobalt concentration was determined after HaCaT cell contamination and chemical toxicity was evaluated in terms of cellular viability and clonogenic survival. We investigated damage to DNA in HaCaT cells by combined treatment with chemical cobalt and a moderate gamma-ray dose. Additive effects of cobalt and irradiation were demonstrated. The underlying mechanism of cobalt toxicity is not clearly established, but our results seem to indicate that the toxicity of Co(II) and of irradiation arises from production of reactive oxygen species.
- Subjects :
- Cell Line
Cell Survival drug effects
Cell Survival radiation effects
Clone Cells
Cobalt metabolism
Comet Assay
DNA Damage
Dose-Response Relationship, Drug
Dose-Response Relationship, Radiation
Gamma Rays
Humans
Keratinocytes metabolism
Reactive Oxygen Species metabolism
Reactive Oxygen Species radiation effects
Risk Assessment
Skin cytology
Skin drug effects
Skin radiation effects
Cobalt toxicity
Cobalt Radioisotopes toxicity
Keratinocytes drug effects
Keratinocytes radiation effects
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3177
- Volume :
- 24
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Toxicology in vitro : an international journal published in association with BIBRA
- Publication Type :
- Academic Journal
- Accession number :
- 19735721
- Full Text :
- https://doi.org/10.1016/j.tiv.2009.08.027