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Two-dimensional LC-MS/MS determination of antiretroviral drugs in rat serum and urine.
- Source :
-
Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2009 Dec 05; Vol. 50 (5), pp. 994-9. Date of Electronic Publication: 2009 Sep 02. - Publication Year :
- 2009
-
Abstract
- A simple, rapid, reliable and highly sensitive on-line two-dimensional reversed-phase liquid chromatography-tandem mass spectrometric (2D-LC/MS/MS) method to determine antiretroviral drugs viz., abacavir (ABC), nevirapine (NVP) and indinavir (IDV) in rat serum and urine was developed and validated. The analytes were extracted on-line from rat serum and urine by a restricted access material (RAM) column and back-flushed into the reversed-phase C18 column for separation by LC. Detection was carried out by ESI-MS/MS. The developed method showed good selectivity, accuracy and precision for quantification of the antiretroviral drugs in rat serum and urine. Quantification limits for abacavir and nevirapine were 4.0 ng ml(-1), whereas for indinavir 4.7 ng ml(-1). The calibration graphs were linear in the range of 4-50 ng ml(-1)for abacavir, nevirapine and indinavir. The method was successfully applied to study the pharmacokinetics of antiretroviral in rats.
- Subjects :
- Animals
Calibration
Dideoxynucleosides blood
Dideoxynucleosides urine
Drug Monitoring methods
Equipment Design
Models, Chemical
Nevirapine blood
Nevirapine urine
Rats
Rats, Wistar
Reproducibility of Results
Spectrometry, Mass, Electrospray Ionization methods
Time Factors
Anti-Retroviral Agents blood
Anti-Retroviral Agents urine
Chromatography, Liquid methods
Tandem Mass Spectrometry methods
Subjects
Details
- Language :
- English
- ISSN :
- 1873-264X
- Volume :
- 50
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of pharmaceutical and biomedical analysis
- Publication Type :
- Academic Journal
- Accession number :
- 19729260
- Full Text :
- https://doi.org/10.1016/j.jpba.2009.07.005