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Identification and functional study of a shrimp Dorsal homologue.

Identification and functional study of a shrimp Dorsal homologue.

Authors :
Huang XD
Yin ZX
Jia XT
Liang JP
Ai HS
Yang LS
Liu X
Wang PH
Li SD
Weng SP
Yu XQ
He JG
Source :
Developmental and comparative immunology [Dev Comp Immunol] 2010 Feb; Vol. 34 (2), pp. 107-13. Date of Electronic Publication: 2009 Sep 08.
Publication Year :
2010

Abstract

Rel/NF-kappaB transcription factors play central roles in induction and regulation of innate immune responses. Here, identification and functional analysis of LvDorsal, a Dorsal homologue from the Pacific white shrimp Litopenaeus vannamei, were described. The full-length cDNA of LvDorsal is 2204bp with an open reading frame that encodes 400 amino acids. The deduced LvDorsal contains a conserved Rel homology domain (RHD), an IPT (Ig-like, plexins and transcription factors) domain and a nucleus localization signal, suggesting that it belongs to the class II NF-kappaB. RT-PCR analysis showed that LvDorsal mRNAs were expressed in all the tissues tested, including gill, epidermis, hemocytes, intestine, stomach, eyestalk, brain, hepatopancreas, muscle, heart and pyloric caecum. Immunofluorescence assay showed that recombinant LvDorsal was translocated into the nucleus of Drosophila S2 cells. Electrophoretic mobility shift assay illustrated that recombinant LvDorsal RHD from S2 cells bound specifically with D. melanogaster kappaB motifs. Additionally, the dual-luciferase reporter assays indicated that LvDorsal could transactivate the reporter gene controlled by the 5' flanking region of shrimp penaeidin-4 and Drosophila attacin genes, suggesting that LvDorsal can regulate the transcription of shrimp penaeidin-4 gene. Study of LvDorsal will help us to better understand shrimp immunity and may help to obtain more effective methods to prevent shrimp diseases.

Details

Language :
English
ISSN :
1879-0089
Volume :
34
Issue :
2
Database :
MEDLINE
Journal :
Developmental and comparative immunology
Publication Type :
Academic Journal
Accession number :
19723535
Full Text :
https://doi.org/10.1016/j.dci.2009.08.009