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Limited heterogeneity of rearranged T-cell receptor V alpha transcripts in brains of multiple sclerosis patients.
- Source :
-
Nature [Nature] 1990 May 24; Vol. 345 (6273), pp. 344-6. - Publication Year :
- 1990
-
Abstract
- The identification of activated T cells in the brain of individuals with multiple sclerosis (MS) indicates that these cells are critical in the pathogenesis of this disease. In an attempt to elucidate the nature of the lymphocytic infiltration, we used the polymerase chain reaction to amplify T-cell antigen receptor (TCR) V alpha sequences from transcripts derived from MS brain lesions. In each of three MS brains, only two to four rearranged TCR V alpha transcripts were detected. No V alpha transcripts could be found in control brains. Sequence analysis of transcripts encoded by the V alpha 12.1 region showed rearrangements to a limited number of J alpha region segments. These results imply that TCR V alpha gene expression in MS brain lesions is restricted.
- Subjects :
- Base Sequence
DNA genetics
Humans
Molecular Sequence Data
Multiple Sclerosis genetics
Nucleic Acid Hybridization
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Restriction Mapping
Brain immunology
Gene Expression
Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor
Multiple Sclerosis immunology
Receptors, Antigen, T-Cell genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0028-0836
- Volume :
- 345
- Issue :
- 6273
- Database :
- MEDLINE
- Journal :
- Nature
- Publication Type :
- Academic Journal
- Accession number :
- 1971424
- Full Text :
- https://doi.org/10.1038/345344a0