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Isolation and characterization of peptides from Momordica cochinchinensis seeds.
- Source :
-
Journal of natural products [J Nat Prod] 2009 Aug; Vol. 72 (8), pp. 1453-8. - Publication Year :
- 2009
-
Abstract
- The plant Momordica cochinchinensis has traditionally been used in Chinese medicine to treat a variety of illnesses. A range of bioactive molecules have been isolated from this plant, including peptides, which are the focus of this study. Here we report the isolation and characterization of two novel peptides, MCoCC-1 and MCoCC-2, containing 33 and 32 amino acids, respectively, which are toxic against three cancer cell lines. The two peptides are highly homologous to one another, but show no sequence similarity to known peptides. Elucidation of the three-dimensional structure of MCoCC-1 suggests the presence of a cystine knot motif, also found in a family of trypsin inhibitor peptides from this plant. However, unlike its structural counterparts, MCoCC-1 does not inhibit trypsin. MCoCC-1 has a well-defined structure, characterized mainly by a triple-stranded antiparallel beta-sheet, but unlike the majority of cystine knot proteins MCoCC-1 contains a disordered loop presumably as a result of flexibility in a localized region of the molecule. Of the cell lines tested, MCoCC-1 is the most toxic against a human melanoma cell line (MM96L) and is nonhemolytic to human erythrocytes. The role of these peptides within the plant remains to be determined.
- Subjects :
- Amino Acid Sequence
Antineoplastic Agents, Phytogenic pharmacology
Drug Screening Assays, Antitumor
Erythrocytes drug effects
Hemolysis drug effects
Humans
Nuclear Magnetic Resonance, Biomolecular
Peptides pharmacology
Peptides, Cyclic pharmacology
Seeds chemistry
Sequence Homology, Amino Acid
Trypsin Inhibitors chemistry
Vietnam
Antineoplastic Agents, Phytogenic chemistry
Antineoplastic Agents, Phytogenic isolation & purification
Momordica chemistry
Peptides chemistry
Peptides isolation & purification
Peptides, Cyclic chemistry
Peptides, Cyclic isolation & purification
Plants, Medicinal chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1520-6025
- Volume :
- 72
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of natural products
- Publication Type :
- Academic Journal
- Accession number :
- 19711988
- Full Text :
- https://doi.org/10.1021/np900174n