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Dual functions of Mss51 couple synthesis of Cox1 to assembly of cytochrome c oxidase in Saccharomyces cerevisiae mitochondria.
- Source :
-
Molecular biology of the cell [Mol Biol Cell] 2009 Oct; Vol. 20 (20), pp. 4371-80. Date of Electronic Publication: 2009 Aug 26. - Publication Year :
- 2009
-
Abstract
- Functional interactions of the translational activator Mss51 with both the mitochondrially encoded COX1 mRNA 5'-untranslated region and with newly synthesized unassembled Cox1 protein suggest that it has a key role in coupling Cox1 synthesis with assembly of cytochrome c oxidase. Mss51 is present at levels that are near rate limiting for expression of a reporter gene inserted at COX1 in mitochondrial DNA, and a substantial fraction of Mss51 is associated with Cox1 protein in assembly intermediates. Thus, sequestration of Mss51 in assembly intermediates could limit Cox1 synthesis in wild type, and account for the reduced Cox1 synthesis caused by most yeast mutations that block assembly. Mss51 does not stably interact with newly synthesized Cox1 in a mutant lacking Cox14, suggesting that the failure of nuclear cox14 mutants to decrease Cox1 synthesis, despite their inability to assemble cytochrome c oxidase, is due to a failure to sequester Mss51. The physical interaction between Mss51 and Cox14 is dependent upon Cox1 synthesis, indicating dynamic assembly of early cytochrome c oxidase intermediates nucleated by Cox1. Regulation of COX1 mRNA translation by Mss51 seems to be an example of a homeostatic mechanism in which a positive effector of gene expression interacts with the product it regulates in a posttranslational assembly process.
- Subjects :
- 5' Untranslated Regions
Electron Transport Complex IV genetics
Genes, Reporter
Genes, Synthetic
Homeostasis
Membrane Proteins physiology
Mitochondrial Proteins physiology
Protein Biosynthesis physiology
Protein Interaction Mapping
Protein Processing, Post-Translational physiology
Protein Subunits
RNA, Fungal genetics
RNA, Fungal metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
Recombinant Fusion Proteins physiology
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae Proteins genetics
Transcription Factors genetics
Electron Transport Complex IV biosynthesis
Gene Expression Regulation, Fungal physiology
Mitochondria enzymology
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins biosynthesis
Saccharomyces cerevisiae Proteins physiology
Transcription Factors physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1939-4586
- Volume :
- 20
- Issue :
- 20
- Database :
- MEDLINE
- Journal :
- Molecular biology of the cell
- Publication Type :
- Academic Journal
- Accession number :
- 19710419
- Full Text :
- https://doi.org/10.1091/mbc.E09-06-0522