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Cytotoxicity of solubilization vehicles for Trichomonas gallinae and Tritrichomonas foetus measured by the resazurin microtiter assay.

Authors :
Duarte M
Giordani RB
Carli GA
Zuanazzi JA
Macedo AJ
Tasca T
Source :
Veterinary parasitology [Vet Parasitol] 2009 Dec 03; Vol. 166 (1-2), pp. 167-70. Date of Electronic Publication: 2009 Jul 29.
Publication Year :
2009

Abstract

The parasites Tritrichomonas foetus and Trichomonas gallinae present veterinary and economic importance since they cause bovine and avian trichomonosis, respectively. The absence of a specific treatment and the necessity of effective and safe drugs against these etiologic agents have stimulated the search for new antiprotozoal drugs with high activity, low toxicity to the animal, and low cost. Screening of potential antiprotozoal molecules is currently a common practice and different kinds of organic solvents and surfactant vehicles are used, since many bioactive compounds have low water solubility. Thus, it is important to determine the toxicity and to provide the minimal inhibitory concentration (MIC) values of the most common solubilization vehicles used in biological activity in vitro evaluation: ethanol, methanol, isopropanol, acetone, DMSO, tween 20 and tween 80. The assays were conducted employing the resazurin microtiter assay, which demonstrated a rapid, safe, and quantitative method for the in vitro determination of T. foetus and T. gallinae trophozoites viability. In summary, all solvents and surfactants, except ethanol, could be used in cytotoxicity assays against T. foetus, and acetone, tween 20 and tween 80 are the preferential vehicles for in vitro analysis of potential bioactive compounds against T. gallinae, though these must be used with caution.

Details

Language :
English
ISSN :
1873-2550
Volume :
166
Issue :
1-2
Database :
MEDLINE
Journal :
Veterinary parasitology
Publication Type :
Academic Journal
Accession number :
19695781
Full Text :
https://doi.org/10.1016/j.vetpar.2009.07.035