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Cytotoxicity of solubilization vehicles for Trichomonas gallinae and Tritrichomonas foetus measured by the resazurin microtiter assay.
- Source :
-
Veterinary parasitology [Vet Parasitol] 2009 Dec 03; Vol. 166 (1-2), pp. 167-70. Date of Electronic Publication: 2009 Jul 29. - Publication Year :
- 2009
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Abstract
- The parasites Tritrichomonas foetus and Trichomonas gallinae present veterinary and economic importance since they cause bovine and avian trichomonosis, respectively. The absence of a specific treatment and the necessity of effective and safe drugs against these etiologic agents have stimulated the search for new antiprotozoal drugs with high activity, low toxicity to the animal, and low cost. Screening of potential antiprotozoal molecules is currently a common practice and different kinds of organic solvents and surfactant vehicles are used, since many bioactive compounds have low water solubility. Thus, it is important to determine the toxicity and to provide the minimal inhibitory concentration (MIC) values of the most common solubilization vehicles used in biological activity in vitro evaluation: ethanol, methanol, isopropanol, acetone, DMSO, tween 20 and tween 80. The assays were conducted employing the resazurin microtiter assay, which demonstrated a rapid, safe, and quantitative method for the in vitro determination of T. foetus and T. gallinae trophozoites viability. In summary, all solvents and surfactants, except ethanol, could be used in cytotoxicity assays against T. foetus, and acetone, tween 20 and tween 80 are the preferential vehicles for in vitro analysis of potential bioactive compounds against T. gallinae, though these must be used with caution.
- Subjects :
- Alcohols toxicity
Animals
Dimethyl Sulfoxide toxicity
Indicators and Reagents toxicity
Metronidazole toxicity
Microbial Sensitivity Tests
Parasitic Sensitivity Tests veterinary
Polysorbates toxicity
Protozoan Infections, Animal parasitology
Trichomonas Infections parasitology
Trophozoites drug effects
Oxazines toxicity
Pharmaceutical Vehicles toxicity
Solvents toxicity
Trichomonas drug effects
Trichomonas Infections veterinary
Tritrichomonas foetus drug effects
Xanthenes toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2550
- Volume :
- 166
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Veterinary parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 19695781
- Full Text :
- https://doi.org/10.1016/j.vetpar.2009.07.035