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Optimization of the non-invasive 13C-sucrose breath test in a rat model of methotrexate-induced mucositis.

Authors :
Tooley KL
Howarth GS
Lymn KA
Butler RN
Source :
Cancer chemotherapy and pharmacology [Cancer Chemother Pharmacol] 2010 Apr; Vol. 65 (5), pp. 913-21. Date of Electronic Publication: 2009 Aug 19.
Publication Year :
2010

Abstract

Purpose: In order to determine the sensitivity and specificity of the test and to optimize experimental conditions utilizing the SBT in a rat model of chemotherapy-induced small intestinal damage.<br />Methods: Initially, a 13C-sucrose dose-response study was performed in rats to determine an optimal sucrose concentration for the SBT; then applied to assess chemotherapy-induced intestinal damage. A further study was conducted to establish a SBT time-course of methotrexate-induced small intestinal damage and repair. Animals were killed at 96 or 144 h.<br />Results: A sucrose concentration of 0.25 g/ml was optimal (20% CV) for reproducibility and detection of intestinal damage. Maximal damage occurred at 72 h, small intestinal repair was initiated by 96 h and continued at 144 h post-MTX, as determined by the SBT and confirmed by biochemical analyses. Levels of sensitivity and specificity for the SBT were 98 and 94%, respectively.<br />Conclusions: The SBT is a reliable non-invasive marker of small intestinal health and damage with a high degree of sensitivity and specificity.

Details

Language :
English
ISSN :
1432-0843
Volume :
65
Issue :
5
Database :
MEDLINE
Journal :
Cancer chemotherapy and pharmacology
Publication Type :
Academic Journal
Accession number :
19690860
Full Text :
https://doi.org/10.1007/s00280-009-1098-2