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Optimization of the non-invasive 13C-sucrose breath test in a rat model of methotrexate-induced mucositis.
- Source :
-
Cancer chemotherapy and pharmacology [Cancer Chemother Pharmacol] 2010 Apr; Vol. 65 (5), pp. 913-21. Date of Electronic Publication: 2009 Aug 19. - Publication Year :
- 2010
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Abstract
- Purpose: In order to determine the sensitivity and specificity of the test and to optimize experimental conditions utilizing the SBT in a rat model of chemotherapy-induced small intestinal damage.<br />Methods: Initially, a 13C-sucrose dose-response study was performed in rats to determine an optimal sucrose concentration for the SBT; then applied to assess chemotherapy-induced intestinal damage. A further study was conducted to establish a SBT time-course of methotrexate-induced small intestinal damage and repair. Animals were killed at 96 or 144 h.<br />Results: A sucrose concentration of 0.25 g/ml was optimal (20% CV) for reproducibility and detection of intestinal damage. Maximal damage occurred at 72 h, small intestinal repair was initiated by 96 h and continued at 144 h post-MTX, as determined by the SBT and confirmed by biochemical analyses. Levels of sensitivity and specificity for the SBT were 98 and 94%, respectively.<br />Conclusions: The SBT is a reliable non-invasive marker of small intestinal health and damage with a high degree of sensitivity and specificity.
- Subjects :
- Animals
Body Weight drug effects
Carbon Isotopes analysis
Disease Models, Animal
Dose-Response Relationship, Drug
Eating drug effects
Female
Intestine, Small metabolism
Organ Size drug effects
Rats
Sensitivity and Specificity
Sucrase metabolism
Antimetabolites, Antineoplastic toxicity
Breath Tests methods
Methotrexate toxicity
Mucositis chemically induced
Sucrose analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0843
- Volume :
- 65
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Cancer chemotherapy and pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 19690860
- Full Text :
- https://doi.org/10.1007/s00280-009-1098-2