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Molecular characterization of Cryptosporidium spp. from fecal samples of birds kept in captivity in Brazil.
- Source :
-
Veterinary parasitology [Vet Parasitol] 2009 Dec 03; Vol. 166 (1-2), pp. 47-51. Date of Electronic Publication: 2009 Jul 29. - Publication Year :
- 2009
-
Abstract
- The aim of this study was to determine the prevalence of Cryptosporidium species and genotypes in birds kept in captivity in Brazil. A total of 966 samples from 18 families of birds was collected and stored in 5% potassium dichromate solution at 4 degrees C until processing. Oocysts were purified in Sheather sugar solution following extraction of genomic DNA. Molecular analyses were performed using nested-PCR for amplification of fragments of the 18S subunit of rRNA gene and of the actin gene. Amplification of Cryptosporidium DNA fragments was obtained in 47 (4.86%) samples. Sequencing of amplified fragments and phylogenetic analyses allowed the identification of Cryptosporidium baileyi in a black vulture (Coragyps atratus), a domestic chicken (Gallus gallus domesticus) and a saffron finch (Sicalis flaveola); Cryptosporidium galli in canaries (Serinus canaria), a cockatiel (Nymphicus hollandicus) and lesser seed-finches (Oryzoborus angolensis); Cryptosporidium meleagridis in a domestic chicken (G. g. domesticus); Cryptosporidium parvum in a cockatiel (N. hollandicus); Cryptosporidium avian genotype I in a canary (S. canaria) and an Indian peafowl (Pavo cristatus); Cryptosporidium avian genotype II in ostriches (Struthio camelus) and Cryptosporidium avian genotype III in a cockatiel (N. hollandicus) and a peach-faced lovebird (Agapornis roseicolis).
- Subjects :
- Actins genetics
Animals
Birds
Brazil
Cryptosporidiosis parasitology
Cryptosporidium classification
Cryptosporidium isolation & purification
Feces parasitology
Phylogeny
RNA, Ribosomal, 18S genetics
Animals, Zoo parasitology
Bird Diseases parasitology
Cryptosporidiosis veterinary
Cryptosporidium genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2550
- Volume :
- 166
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Veterinary parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 19683397
- Full Text :
- https://doi.org/10.1016/j.vetpar.2009.07.033