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Comprehensive proteomic analysis of human endometrial fluid aspirate.
- Source :
-
Journal of proteome research [J Proteome Res] 2009 Oct; Vol. 8 (10), pp. 4622-32. - Publication Year :
- 2009
-
Abstract
- The endometrial fluid is a noninvasive sample which contains numerous secreted proteins representative of endometrial function and reflects the state of the endometrium. In this study, we describe, for the first time, a comprehensive catalogue of proteins of the endometrial fluid during the secretory phase of the menstrual cycle. To achieve this objective, three different but complementary strategies were used: First, in-solution digestion followed by reverse phase high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS); second, protein separation by denaturing one-dimensional electrophoresis (SDS-PAGE) followed by HPLC-MS/MS analysis. Finally, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) followed by MALDI-TOF/TOF analysis. The combination of the three strategies led to the successful identification of 803 different proteins in the International Protein Index (IPI) human database (v3.48). An extensive description of the endometrial fluid proteome will help provide the basis for a better understanding of a number of diseases and processes, including endometriosis, endometrial cancer and embryo implantation. We believe that the thorough catalogue of proteins presented here can serve as a valuable reference for the study of embryo implantation and for future biomarker discovery involved in pathologic alterations of endometrial function.
- Subjects :
- Adolescent
Adult
Amino Acid Sequence
Biopsy, Needle
Chromatography, High Pressure Liquid
Cluster Analysis
Electrophoresis, Gel, Two-Dimensional
Embryo Implantation
Endometrial Neoplasms
Endometriosis
Female
Humans
Isoelectric Focusing
Luteal Phase metabolism
Middle Aged
Molecular Sequence Data
Mucins metabolism
Peptides
Tandem Mass Spectrometry
Trefoil Factor-3
Body Fluids chemistry
Endometrium metabolism
Proteome analysis
Proteomics methods
Subjects
Details
- Language :
- English
- ISSN :
- 1535-3907
- Volume :
- 8
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of proteome research
- Publication Type :
- Academic Journal
- Accession number :
- 19670903
- Full Text :
- https://doi.org/10.1021/pr9004426