Back to Search
Start Over
Association of scavenger receptors in adipose tissue with insulin resistance in nondiabetic humans.
- Source :
-
Arteriosclerosis, thrombosis, and vascular biology [Arterioscler Thromb Vasc Biol] 2009 Sep; Vol. 29 (9), pp. 1328-35. Date of Electronic Publication: 2009 Aug 10. - Publication Year :
- 2009
-
Abstract
- Objective: Scavenger receptors play crucial roles in the pathogenesis of atherosclerosis, but their role in insulin resistance has not been explored. We hypothesized that scavenger receptors are present in human adipose tissue resident macrophages, and their gene expression is regulated by adiponectin and thaizolidinediones.<br />Methods and Results: The gene expression of scavenger receptors including scavenger receptor-A (SRA), CD36, and lectin-like oxidized LDL receptor-1 (LOX-1) were studied in subcutaneous adipose tissue of nondiabetic subjects and in vitro. Adipose tissue SRA expression was independently associated with insulin resistance. Pioglitazone downregulated SRA gene expression in adipose tissue of subjects with impaired glucose tolerance and decreased LOX-1 mRNA in vitro. Macrophage LOX-1 expression was decreased when macrophages were cocultured with adipocytes or when exposed to adipocyte conditioned medium. Adding adiponectin neutralizing antibody resulted in a 2-fold increase in LOX-1 gene expression demonstrating that adiponectin regulates LOX-1 expression.<br />Conclusions: Adipose tissue scavenger receptors are strongly associated with insulin resistance. Pioglitazone and adiponectin regulate gene expression of SRA and LOX-1, and this may have clinical implications in arresting the untoward sequalae of insulin resistance and diabetes, including accelerated atherosclerosis.
- Subjects :
- Adipocytes metabolism
Adiponectin metabolism
Adult
Aged
CD36 Antigens drug effects
Cell Line
Coculture Techniques
Down-Regulation
Female
Humans
Macrophages metabolism
Male
Middle Aged
Obesity metabolism
Obesity physiopathology
Pioglitazone
RNA, Messenger metabolism
Receptors, Scavenger genetics
Receptors, Scavenger metabolism
Scavenger Receptors, Class A drug effects
Scavenger Receptors, Class E drug effects
Subcutaneous Fat metabolism
Treatment Outcome
Young Adult
Adipocytes drug effects
Hypoglycemic Agents therapeutic use
Insulin Resistance
Macrophages drug effects
Metformin therapeutic use
Obesity drug therapy
Receptors, Scavenger drug effects
Subcutaneous Fat drug effects
Thiazolidinediones therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1524-4636
- Volume :
- 29
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Arteriosclerosis, thrombosis, and vascular biology
- Publication Type :
- Academic Journal
- Accession number :
- 19667111
- Full Text :
- https://doi.org/10.1161/ATVBAHA.109.186957