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Poly(ADP-ribosyl)ation directs recruitment and activation of an ATP-dependent chromatin remodeler.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2009 Aug 18; Vol. 106 (33), pp. 13770-4. Date of Electronic Publication: 2009 Aug 06. - Publication Year :
- 2009
-
Abstract
- Posttranslational modifications play a key role in recruiting chromatin remodeling and modifying enzymes to specific regions of chromosomes to modulate chromatin structure. Alc1 (amplified in liver cancer 1), a member of the SNF2 ATPase superfamily with a carboxy-terminal macrodomain, is encoded by an oncogene implicated in the pathogenesis of hepatocellular carcinoma. Here we show that Alc1 interacts transiently with chromatin-associated proteins, including histones and the poly(ADP-ribose) polymerase Parp1. Alc1 ATPase and chromatin remodeling activities are strongly activated by Parp1 and its substrate NAD and require an intact macrodomain capable of binding poly(ADP-ribose). Alc1 is rapidly recruited to nucleosomes in vitro and to chromatin in cells when Parp1 catalyzes PAR synthesis. We propose that poly(ADP-ribosyl)ation of chromatin-associated Parp1 serves as a mechanism for targeting a SNF2 family remodeler to chromatin.
- Subjects :
- Adenosine Triphosphatases chemistry
Carcinoma, Hepatocellular metabolism
Cell Line
Cell Line, Tumor
Chromatin chemistry
Chromatin Assembly and Disassembly
DNA Helicases chemistry
DNA-Binding Proteins chemistry
HeLa Cells
Humans
Liver Neoplasms metabolism
Nucleosomes metabolism
Protein Processing, Post-Translational
Protein Structure, Tertiary
Transcription Factors chemistry
Adenosine Triphosphate chemistry
DNA Helicases physiology
DNA-Binding Proteins physiology
Poly(ADP-ribose) Polymerases chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1091-6490
- Volume :
- 106
- Issue :
- 33
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 19666485
- Full Text :
- https://doi.org/10.1073/pnas.0906920106