Back to Search
Start Over
Enhanced effects of BMP-binding peptide combined with recombinant human BMP-2 on the healing of a rodent segmental femoral defect.
- Source :
-
Journal of orthopaedic research : official publication of the Orthopaedic Research Society [J Orthop Res] 2010 Feb; Vol. 28 (2), pp. 258-64. - Publication Year :
- 2010
-
Abstract
- BMP-binding peptide (BBP) enhances the osteogenic activity of recombinant human bone morphogenetic protein-2 (rhBMP-2), but the mechanism underlying the enhancement remains unclear. We aimed to elucidate the potential enhanced efficacy of BBP using critical-sized segmental femoral bone defects in rats. Seventy defects in seven groups of rats were filled with various amounts (0, 2, 5, and 10 microg) of rhBMP-2 with or without 1000 microg BBP. Radiographs were obtained after 4 and 8 weeks. The animals were euthanized at 8 weeks, and femoral specimens were assessed manually, evaluated for bone volume using microcomputed tomography, and subjected to histological or biomechanical analysis. Although 10 microg rhBMP-2 yielded consistent results in terms of bone healing and quality of bone repair across the segmental defect, lower doses of rhBMP-2 failed to induce satisfactory bone healing. However, the combined administration of lower doses of rhBMP-2 and BBP induced the formation of significantly large amounts of bone. Our results suggest that the combined administration of rhBMP-2 and BBP facilitates bone healing and has potential clinical applications.<br /> ((c) 2009 Orthopaedic Research Society.)
- Subjects :
- Animals
Bone Morphogenetic Protein 2
Bone Regeneration drug effects
Dose-Response Relationship, Drug
Drug Therapy, Combination methods
Femur diagnostic imaging
Femur drug effects
Fracture Healing drug effects
Fractures, Bone diagnostic imaging
Male
Models, Animal
Rats
Rats, Inbred Lew
Treatment Outcome
X-Ray Microtomography
Bone Morphogenetic Proteins administration & dosage
Femur injuries
Fractures, Bone drug therapy
Recombinant Proteins administration & dosage
Transforming Growth Factor beta administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1554-527X
- Volume :
- 28
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of orthopaedic research : official publication of the Orthopaedic Research Society
- Publication Type :
- Academic Journal
- Accession number :
- 19639633
- Full Text :
- https://doi.org/10.1002/jor.20970