[Identification of mycobacteria by high-performance liquid chromatography].

Authors :: Makarova MV
Krasnova MA
Moroz AM
Source :: Zhurnal mikrobiologii, epidemiologii i immunobiologii [Zh Mikrobiol Epidemiol Immunobiol] 2009 May-Jun (3), pp. 64-6.
Publication Year :: 2009
Abstract: Aim: To compare results of mycobacteria identification by bacteriologic methods as well as by high-performance liquid chromatography (HPLC).<br />Materials and Methods: Two hundred and eighty strains of mycobacteria isolated from respiratory specimens and identified by bacteriologic methods and HPLC were studied.<br />Results: It was established that results of HLPC use were highly correlated with results of microbiologic methods of mycobacteria identification: for identification of M. tuberculosis complex the correlation was 97.0%, for nontuberculous (NTM) slowly growing mycobacteria--95.3%, for quickly growing NTM--96.2% (overall--96.1%). Results of identification of mycobacteria by HPLC were ready in significantly shorter time-frame (during 24 hours).<br />Conclusion: HLPC method could be recommended for identification of mycobacteria in bacteriologic reference laboratories.