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Halobacillus hunanensis sp. nov., a moderately halophilic bacterium isolated from a subterranean brine.
- Source :
-
Antonie van Leeuwenhoek [Antonie Van Leeuwenhoek] 2009 Nov; Vol. 96 (4), pp. 497-504. Date of Electronic Publication: 2009 Jul 18. - Publication Year :
- 2009
-
Abstract
- A moderately halophilic, Gram-positive, catalase- and oxidase-positive, rod-shaped, aerobic bacterium, designated strain JSM 071077(T), was isolated from a subterranean brine sample collected from a salt mine in Hunan Province, China. Cells were motile by means of peritrichous flagella and formed ellipsoidal endospores lying in subterminal swollen sporangia. Strain JSM 071077(T) was able to grow with 2-25% (w/v) total salts (optimum, 5-10%), at pH 6.0-10.0 (optimum, pH 7.5) and 10-40 degrees C (optimum, 25-30 degrees C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The predominant menaquinone was MK-7, and the major cellular fatty acids were anteiso-C(15:0), anteiso-C(17:0) and iso-C(15:0). The genomic DNA G+C content was 41.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequence comparisons revealed that strain JSM 071077(T) should be assigned to the genus Halobacillus, being related most closely to the type strain of Halobacillus naozhouensis (98.8% sequence similarity), and the two strains formed a distinct subline in the neighbor-joining, minimum-evolution and maximum-parsimony phylogenetic trees. The sequence similarities between the novel isolate and the type strains of other recognized Halobacillus species ranged from 97.6% (with Halobacillus alkaliphilus) to 95.2% (with Halobacillus kuroshimensis). The results of the phylogenetic analyses, combined with DNA-DNA relatedness data, phenotypic characteristics and chemotaxonomic information, support that strain JSM 071077(T) represents a new species of the genus Halobacillus, for which the name Halobacillus hunanensis sp. nov. is proposed. The type strain is JSM 071077(T) (=DSM 21184(T) = KCTC 13235(T)).
- Subjects :
- Aerobiosis
Bacillaceae genetics
Bacillaceae physiology
Base Composition
Catalase metabolism
Cell Wall chemistry
China
Cluster Analysis
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
Diaminopimelic Acid analysis
Fatty Acids analysis
Flagella physiology
Hydrogen-Ion Concentration
Locomotion
Molecular Sequence Data
Oxidoreductases metabolism
Phylogeny
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Spores, Bacterial cytology
Vitamin K 2 analysis
Bacillaceae classification
Bacillaceae isolation & purification
Salts metabolism
Soil Microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1572-9699
- Volume :
- 96
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Antonie van Leeuwenhoek
- Publication Type :
- Academic Journal
- Accession number :
- 19618289
- Full Text :
- https://doi.org/10.1007/s10482-009-9365-0