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Microtexture of larval shell of oyster, Crassostrea nippona: a FIB-TEM study.

Authors :
Kudo M
Kameda J
Saruwatari K
Ozaki N
Okano K
Nagasawa H
Kogure T
Source :
Journal of structural biology [J Struct Biol] 2010 Jan; Vol. 169 (1), pp. 1-5. Date of Electronic Publication: 2009 Jul 16.
Publication Year :
2010

Abstract

The initial formation and subsequent development of larval shells in marine bivalve, Crassostrea nippona were investigated using the FIB-TEM technique. Fourteen hours after fertilization (the trochophore stage), larvae form an incipient shell of 100-150nm thick with a columnar contrast. Selected-area electron diffraction analysis showed a single-crystal aragonite pattern with the c-axis perpendicular to the shell surface. Plan-view TEM analysis suggested that the shell contains high density of {110} twins, which are the origin of the columnar contrast in the cross-sectional images. 72h after fertilization (the veliger stage), the shell grows up to 1.2-1.4mum thick accompanying an additional granular layer between the preexisting layer and embryo to form a distinctive two-layer structure. The granular layer is also composed of aragonite crystals sharing their c-axes perpendicular to the shell surface, but the crystals are arranged with a flexible rotation around the c-axes and not restricted solely to the {110} twin relation. No evidence to suggest the existence of amorphous calcium carbonate (ACC) was found through the observation. The well-regulated crystallographic properties found in the present sample imply initial shell formation probably via a direct deposition of crystalline aragonite.<br /> (Copyright (c) 2009 Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1095-8657
Volume :
169
Issue :
1
Database :
MEDLINE
Journal :
Journal of structural biology
Publication Type :
Academic Journal
Accession number :
19616099
Full Text :
https://doi.org/10.1016/j.jsb.2009.07.014