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Effect of mechanical stretching on expressions of muscle specific transcription factors MyoD, Myf-5, myogenin and MRF4 in proliferated myoblasts.
- Source :
-
Anatomia, histologia, embryologia [Anat Histol Embryol] 2009 Aug; Vol. 38 (4), pp. 305-10. - Publication Year :
- 2009
-
Abstract
- We examined expression of four important members of myogenic regulatory factors (MRFs) in the myoblasts both at mRNA and protein levels, which were subjected to mechanical stretching in in vitro condition. Our results showed that MyoD expression existed both in the stretch and in the control group at all time periods of the mechanical stimulus. Myf-5 expressed only at early stage of the stretch group. Although mRNA and protein expressions of myogenin and MRF4 were detected both in the stretch and in the control group at 12 h after the stretching, their expressions were only shown in the stretch group at 24 h after the mechanical stimulus. However, at 36 and 48 h, none of the MRFs examined except MyoD appeared in both groups. Our results suggest that the MRFs are up-regulated upon mechanical stimulus and each member plays a different major role for either proliferation or differentiation of the myoblasts.
- Subjects :
- Animals
Blotting, Western
Cell Count
Cell Line
Cell Proliferation
Gene Expression
Mice
Microscopy, Phase-Contrast
MyoD Protein genetics
Myoblasts cytology
Myogenic Regulatory Factor 5 genetics
Myogenic Regulatory Factors genetics
Myogenin genetics
RNA, Messenger analysis
Reverse Transcriptase Polymerase Chain Reaction
Stress, Mechanical
MyoD Protein biosynthesis
Myoblasts physiology
Myogenic Regulatory Factor 5 biosynthesis
Myogenic Regulatory Factors biosynthesis
Myogenin biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1439-0264
- Volume :
- 38
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Anatomia, histologia, embryologia
- Publication Type :
- Academic Journal
- Accession number :
- 19614631
- Full Text :
- https://doi.org/10.1111/j.1439-0264.2009.00945.x