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An amperometric D-amino acid biosensor prepared with a thermostable D-proline dehydrogenase and a carbon nanotube-ionic liquid gel.

Authors :
Tani Y
Itoyama Y
Nishi K
Wada C
Shoda Y
Satomura T
Sakuraba H
Ohshima T
Hayashi Y
Yabutani T
Motonaka J
Source :
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry [Anal Sci] 2009 Jul; Vol. 25 (7), pp. 919-23.
Publication Year :
2009

Abstract

Carbon nanotube (CNT) gel, which is composed of a mixture of single-wall CNT, an ionic liquid, and a thermostable D-proline dehydrogenase (D-Pro DH) immobilized electrode was utilized for the determination of D-amino acids (DAAs) in food samples. When a critical comparison with CNT, Ketjen Black (KB), and carbon powder (CP) was also carried out, the CNT/D-Pro DH immobilized electrode showed the highest sensitivity and the lowest detection limit of D-proline. In addition, the CNT/D-Pro DH immobilized electrode was applied to detection of DAAs in rice wine and vinegar samples. The concentrations of DAAs in rice wine and vinegar samples were 0.0210 +/- 0.0001 and 0.55 +/- 0.05 mmol L(-1), respectively.

Details

Language :
English
ISSN :
1348-2246
Volume :
25
Issue :
7
Database :
MEDLINE
Journal :
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
Publication Type :
Academic Journal
Accession number :
19609033
Full Text :
https://doi.org/10.2116/analsci.25.919