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Vascular endothelium expresses 3-mercaptopyruvate sulfurtransferase and produces hydrogen sulfide.
- Source :
-
Journal of biochemistry [J Biochem] 2009 Nov; Vol. 146 (5), pp. 623-6. Date of Electronic Publication: 2009 Jul 15. - Publication Year :
- 2009
-
Abstract
- Hydrogen sulfide (H(2)S) has been recognized as a smooth muscle relaxant. Cystathionine gamma-lyase, which is localized to smooth muscle, is thought to be the major H(2)S-producing enzyme in the thoracic aorta. Here we show that 3-mercaptopyruvate sulfurtransferase (3MST) and cysteine aminotransferase (CAT) are localized to vascular endothelium in the thoracic aorta and produce H(2)S. Both 3MST and CAT were localized to endothelium. Lysates of vascular endothelial cells produced H(2)S from cysteine and alpha-ketoglutarate. The present study provides a new insight into the production and release of H(2)S as a smooth muscle relaxant from vascular endothelium.
- Subjects :
- Animals
Aorta, Thoracic cytology
Aorta, Thoracic enzymology
Blotting, Western
Cystathionine beta-Synthase metabolism
Cystathionine gamma-Lyase metabolism
Endothelial Cells cytology
Endothelial Cells enzymology
Endothelium, Vascular cytology
Immunohistochemistry
Protein Transport
Rats
Endothelium, Vascular enzymology
Hydrogen Sulfide metabolism
Sulfurtransferases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1756-2651
- Volume :
- 146
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 19605461
- Full Text :
- https://doi.org/10.1093/jb/mvp111