[Expression of the voltage-dependent anion channel gene in human ejaculated spermatozoa].

Authors :: Xu X
Wang Y
Yu Z
Chen J
Guo M
Gui YT
Cai ZM
Source :: Zhonghua nan ke xue = National journal of andrology [Zhonghua Nan Ke Xue] 2009 Jun; Vol. 15 (6), pp. 493-8.
Publication Year :: 2009
Abstract: Objective: To identify the genes involved in sperm motility.<br />Methods: We hybridized asthenospermia and normal motile sperm cDNA samples with the human whole genome Affymetrix chip to screen differentially expressed genes. Then we detected the mRNA expressions of the voltage-dependent anion channel genes (VDACs) in human organs and spermatozoa by RT-PCR and compared their expressions in the poor and normal motility spermatozoa.<br />Results: Differentially expressed genes VDACs were identified by analysis of the hybridization signals, including the 3 subtypes VDAC1, VDAC2 and VDAC3. The expression of VDAC2 mRNA was significantly decreased in the poor motility sperm (0.568 +/- 0.036), as compared with the healthy men (0.803 +/- 0.043, P < 0.01).<br />Conclusion: The decreased expression of VDAC2 in the ejaculated spermatozoa is possibly associated with the reduction of sperm motility.

Subjects :: Comparative Genomic Hybridization
Gene Expression
Humans
Infertility, Male metabolism
Male
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Sperm Motility
Infertility, Male genetics
Spermatozoa metabolism
Voltage-Dependent Anion Channel 2 genetics

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