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Decolourization and detoxification of pulp and paper mill effluent by Emericella nidulans var. nidulans.
- Source :
-
Journal of hazardous materials [J Hazard Mater] 2009 Nov 15; Vol. 171 (1-3), pp. 619-25. Date of Electronic Publication: 2009 Jun 18. - Publication Year :
- 2009
-
Abstract
- In this study geno-toxicity analysis along with effluent treatment was taken up to evaluate the efficiency of biological treatment process for safe disposal of treated effluent. Four fungi were isolated from sediments of pulp and paper mill in which PF4 reduced colour (30%) and lignin content (24%) of the effluent on 3rd day. The fungal strain was identified as Emericella nidulans var. nidulans (anamorph: Aspergillus nidulans) on the basis of rDNA ITS1 and rDNA ITS2 region sequences. The process of decolourization was optimized by Taguchi approach. The optimum conditions were temperature (30-35 degrees C), rpm (125), dextrose (0.25%), tryptone (0.1%), inoculum size (7.5%), pH (5) and duration (24h). Decolourization of effluent improved by 31% with reduction in colour (66.66%) and lignin (37%) after treatment by fungi in shake flask. Variation in pH from 6 to 5 had most significant effect on decolourization (71%) while variation in temperature from 30 to 35 degrees C had no effect on the process. Treated effluent was further evaluated for geno-toxicity by alkaline single cell gel electrophoresis (SCGE) assay using Saccharomyces cerevisiae MTCC 36 as model organism, indicated 60% reduction.
- Subjects :
- Biodegradation, Environmental
Bioreactors
Comet Assay
Hydrogen-Ion Concentration
Industrial Waste
Lignin analysis
Paper
Saccharomyces cerevisiae metabolism
Temperature
Time Factors
Waste Disposal, Fluid methods
Water Pollutants, Chemical analysis
Water Purification methods
Aspergillus nidulans metabolism
Emericella metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3336
- Volume :
- 171
- Issue :
- 1-3
- Database :
- MEDLINE
- Journal :
- Journal of hazardous materials
- Publication Type :
- Academic Journal
- Accession number :
- 19586717
- Full Text :
- https://doi.org/10.1016/j.jhazmat.2009.06.041