Back to Search
Start Over
Persistent adeno-associated virus 2 and parvovirus B19 sequences in post-mortem human cerebellum.
- Source :
-
Cerebellum (London, England) [Cerebellum] 2009 Dec; Vol. 8 (4), pp. 490-8. Date of Electronic Publication: 2009 Jul 08. - Publication Year :
- 2009
-
Abstract
- We previously reported in a large cohort (N = 104) of post-mortem tissues the detection of both the non-pathogenic adeno-associated virus (AAV2) in approximately 13% and the pathogenic human parvovirus B19 (B19) in approximately 42% of human brains, particularly the dorsolateral prefrontal cortex. Multiple animal parvoviruses target the developing cerebellum (CBLM) resulting in hypoplasia and ataxia, but very little is known about the human parvoviruses and their ability to infect or cause disease in the CBLM. We have now confirmed in the above cohort the presence of AAV2 and B19 sequences in the CBLM. Our results show that approximately 27% and approximately 70% of human CBLM are positive by nested polymerase chain reaction for AAV2 and B19 sequences, respectively. We also document in a second cohort (N = 10) the presence of AAV2 (50%) and B19 (100%) sequences in the CBLM and correlate our results for B19 with studies from matched sera. Eighty percent (80%) of this cohort was positive for anti-B19 IgG, while none were IgM+, suggesting that most individuals had been previously infected with B19 but none acutely. To our knowledge, this study is the first to demonstrate that both AAV2 and B19 sequences are present at relatively high frequencies in the CBLM and are likely due to persistent rather than acute infection. Further studies will lead to insights into AAV2- and/or B19-CBLM interactions including mechanisms of infection, persistence, and possibly neuropathology, including cerebellar hypoplasia and ataxia.
- Subjects :
- Aged
Aged, 80 and over
Cerebellar Diseases immunology
Cerebellum metabolism
Chi-Square Distribution
Cohort Studies
DNA, Viral genetics
Dependovirus immunology
Enzyme-Linked Immunosorbent Assay methods
Female
Humans
In Vitro Techniques
Middle Aged
Parvovirus B19, Human immunology
Postmortem Changes
Cerebellar Diseases metabolism
Cerebellar Diseases virology
Cerebellum virology
Dependovirus genetics
Parvovirus B19, Human genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1473-4230
- Volume :
- 8
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Cerebellum (London, England)
- Publication Type :
- Academic Journal
- Accession number :
- 19585179
- Full Text :
- https://doi.org/10.1007/s12311-009-0126-4