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Internal ribosome-binding site directs expression of parathyroid hormone analogue (8-84) in Escherichia coli.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1991 Nov 27; Vol. 181 (1), pp. 481-5. - Publication Year :
- 1991
-
Abstract
- Expression of the human parathyroid hormone (PTH) gene in E. coli yielded intact PTH and PTH-(8-84). To determine if PTH-(8-84) is the result of a competing translation initiated from methionine codon-8 or degradation of the intact PTH, twelve new gene constructs with or without an internal ribosome-binding site (iRBS) in the PTH-(1-5) region were prepared via substitution with degenerate codons. Expression of constructs without iRBS produced only intact PTH. Constructs with weak iRBS, including one that resembles the cDNA sequence, yielded PTH-(8-84) as a minor product. In contrast, constructs with strong iRBS produced predominantly or exclusively this shorter analogue.
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 181
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 1958216
- Full Text :
- https://doi.org/10.1016/s0006-291x(05)81444-5