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Determination of kappa and lambda light chains in serum immunoglobulins G, A and M.
- Source :
-
Annals of clinical biochemistry [Ann Clin Biochem] 1991 Sep; Vol. 28 ( Pt 5), pp. 461-6. - Publication Year :
- 1991
-
Abstract
- An enzyme-linked immunosorbent assay is described for measuring kappa and lambda light chains within each of the serum immunoglobulin classes G, A and M. The detection limit was 0.06 U/L for total IgG, IgG kappa and IgG lambda, 0.2 U/L for total IgA, IgA kappa and IgA lambda and 0.5 U/L for total IgM, IgM kappa and IgM lambda. The concentrations of kappa plus lambda light chains from the three different immunoglobulins correlated well within those of total immunoglobulin G, A and M as measured by enzyme-linked immunosorbent assay or by immunonephelometry. Adult values for the kappa/lambda light chain ratio were found to be 2.0 for IgG kappa/lambda, 1.1 for IgA kappa/lambda and 1.7 for IgM kappa/lambda.
- Subjects :
- Adolescent
Adult
Child
Child, Preschool
Enzyme-Linked Immunosorbent Assay methods
Female
Humans
Immunoglobulin A blood
Immunoglobulin G blood
Immunoglobulin M blood
Infant
Infant, Newborn
Male
Reproducibility of Results
Sensitivity and Specificity
Immunoglobulin kappa-Chains blood
Immunoglobulin lambda-Chains blood
Subjects
Details
- Language :
- English
- ISSN :
- 0004-5632
- Volume :
- 28 ( Pt 5)
- Database :
- MEDLINE
- Journal :
- Annals of clinical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 1958048
- Full Text :
- https://doi.org/10.1177/000456329102800507