Effect of polymerization conditions on morphology and chromatographic characteristics of polyacrylamide-based beds (monoliths) for capillary electrochromatography and capillary liquid chromatography.

Pioneering research work on continuous or monolithic beds for LC was published in 1989. However, till date there is a lack of complex investigation of various factors affecting the morphology of the beds polymerized from water-soluble co-monomers. In this article the effect of buffer, used as polymerization medium, concentration and pH, and total monomer concentration on the morphology of the resultant continuous beds are discussed. Evaluation of the continuous beds was performed by means of capillary LC, CEC, and inverse size-exclusion chromatography.