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The reduction of Pseudomonas cytochrome c551 oxidase by chromous ions.
- Source :
-
The Biochemical journal [Biochem J] 1977 Jun 01; Vol. 163 (3), pp. 629-32. - Publication Year :
- 1977
-
Abstract
- The reduction of cytochrome c551 oxidase from Pseudomonas aeruginosa by Cr2+ ions was followed in the stopped-flow apparatus at a number of wavelengths. The c-haem reduction proceeded in a biphasic fashion with second-order rate constants of 2.6 X 10(5)M-1-S-1 and 4.8 X 10(4)M-1-S-1 at 25 degrees C, whereas the biphasic reduction of the d1-haem appeared to be independent of reductant concentration with rate constants of approx. 1.0S-1 and 0.25S-1 respectively. The kinetically determined difference spectra (reduced minus oxidized) for the c- and d1-haems are presented.
Details
- Language :
- English
- ISSN :
- 0264-6021
- Volume :
- 163
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The Biochemical journal
- Publication Type :
- Academic Journal
- Accession number :
- 195577
- Full Text :
- https://doi.org/10.1042/bj1630629