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Stress-related variation in antioxidative enzymes activity and cell metabolism efficiency associated with embryogenesis induction in isolated microspore culture of triticale (x Triticosecale Wittm.).
- Source :
-
Plant cell reports [Plant Cell Rep] 2009 Aug; Vol. 28 (8), pp. 1279-87. Date of Electronic Publication: 2009 Jun 24. - Publication Year :
- 2009
-
Abstract
- Isolated microspore cultures of two spring triticale (x Triticosecale Wittm.) cultivars were used to examine the effect of various stress treatments (either high--32 degrees C or low--5 degrees C temperature with or without nitrogen/carbohydrate starvation) applied to excised anthers on the effectiveness of microspore embryogenesis induction. To quantify the effects of pretreatment conditions, the activity of antioxidative enzymes (catalase, peroxidase and superoxide dismutase) together with respiration rate and heat emission were measured. It was observed that heat shock treatment applied as the only one stress factor increased the activity of antioxidative enzymes which suggests intensive generation of reactive oxygen species. Such pretreatment effectively triggered microspore reprogramming but drastically decreased microspore viability. After low temperature treatment, the activity of antioxidative enzymes was similar to the control subjected only with the stress originated from the transfer to in vitro culture conditions. This pretreatment decreased the number of microspores entering embryogenesis but sustained cell viability and this effect prevailed in the final estimation of microspore embryogenesis effectiveness. For both, low- and high-temperature treatments, interaction with starvation stress was beneficial increasing microspore viability (at 5 degrees C) or efficiency of embryogenesis induction (at 32 degrees C). The latter treatment significantly reduced cell metabolic activity. Physiological background of these effects seems to be different and some hypothetical explanations have been discussed. Received data indicate that in triticale, anther preculture conditions could generate oxidative stress and change the cell metabolic activity which could next be reflected in the cell viability and the efficiency of microspore embryogenesis.
- Subjects :
- Carbohydrate Metabolism
Cell Respiration
Cells, Cultured
Cold Temperature
Edible Grain embryology
Edible Grain enzymology
Flowers embryology
Flowers genetics
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Genes, Plant
Hot Temperature
Nitrogen metabolism
Regeneration
Stress, Physiological
Edible Grain genetics
Embryonic Development
Flowers enzymology
Oxidative Stress
Subjects
Details
- Language :
- English
- ISSN :
- 1432-203X
- Volume :
- 28
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Plant cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 19551385
- Full Text :
- https://doi.org/10.1007/s00299-009-0730-2