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Generation and characterization of human insulin-releasing cell lines.
- Source :
-
BMC cell biology [BMC Cell Biol] 2009 Jun 19; Vol. 10, pp. 49. Date of Electronic Publication: 2009 Jun 19. - Publication Year :
- 2009
-
Abstract
- Background: The in vitro culture of insulinomas provides an attractive tool to study cell proliferation and insulin synthesis and secretion. However, only a few human beta cell lines have been described, with long-term passage resulting in loss of insulin secretion. Therefore, we set out to establish and characterize human insulin-releasing cell lines.<br />Results: We generated ex-vivo primary cultures from two independent human insulinomas and from a human nesidioblastosis, all of which were cultured up to passage number 20. All cell lines secreted human insulin and C-peptide. These cell lines expressed neuroendocrine and islets markers, confirming the expression profile found in the biopsies. Although all beta cell lineages survived an anchorage independent culture, none of them were able to invade an extracellular matrix substrate.<br />Conclusion: We have established three human insulin-releasing cell lines which maintain antigenic characteristics and insulin secretion profiles of the original tumors. These cell lines represent valuable tools for the study of molecular events underlying beta cell function and dysfunction.
- Subjects :
- Adult
Biomarkers metabolism
Cell Adhesion Molecules genetics
Cell Adhesion Molecules physiology
Cell Line, Tumor
Cell Movement physiology
Cell Proliferation
Female
Humans
Insulin-Secreting Cells metabolism
Insulinoma
Male
Nesidioblastosis
Pancreatic Neoplasms
Peptide Hydrolases metabolism
RNA, Messenger metabolism
Young Adult
Cell Line
Insulin metabolism
Insulin-Secreting Cells cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2121
- Volume :
- 10
- Database :
- MEDLINE
- Journal :
- BMC cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 19545371
- Full Text :
- https://doi.org/10.1186/1471-2121-10-49