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Inhibition of uropathogens by lactic acid bacteria isolated from dairy foods and cow's intestine in western Nigeria.
- Source :
-
Archives of microbiology [Arch Microbiol] 2009 Aug; Vol. 191 (8), pp. 639-48. Date of Electronic Publication: 2009 Jun 16. - Publication Year :
- 2009
-
Abstract
- A total of 96 lactic acid bacteria (LAB) were isolated from African indigenous fermented products and cow's intestines to study their inhibitory capability against multi-drug-resistant uropathogens. Escherichia coli accounted for approximately 45% of isolated uropathogens, followed by Staphylococcus spp. (20%). The Gram negative uropathogens were highly resistant to quinolones, co-trimoxazole, teicoplanin and some beta-lactams, while the Staphylococcus spp. showed high resistance to aminoglycosides, beta-lactams and macrolides. Twenty-four LAB isolates were selected based on their antimicrobial activity against two uropathogenic Staphylococcus aureus strains and bacteriocin production. LAB strains showing antimicrobial activity were grouped into smaller groups through amplified ribosomal DNA restriction analysis (ARDRA). Representative strains were identified as Weissella spp., Enterococcus faecium, Lactococcus lactis and Lactobacillus brevis through sequencing of 16S rDNA. The Weissella spp. and L. brevis strains demonstrated remarkable inhibitory activity against seven strains of Gram negative uropathogens. Two strains of L. lactis produced a bacteriocin-like inhibitory substance active against Lactobacillus sakei. In this study, an unusual high rate of co-trimoxazole, quinolones and macrolides resistance among uropathogens from south west Nigeria was discovered. Based on their sensitivity to Weissella spp., there is a potential for using these LAB as a natural approach for the protection against the uropathogens assayed.
- Subjects :
- Animals
Antibiosis
Bacteriocins biosynthesis
DNA, Bacterial genetics
Drug Resistance, Bacterial
Escherichia coli genetics
Escherichia coli isolation & purification
Escherichia coli pathogenicity
Food Microbiology
Humans
Lactobacillus genetics
Lactobacillus metabolism
Lactobacillus physiology
Lactococcus lactis genetics
Lactococcus lactis metabolism
Lactococcus lactis physiology
Nigeria
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Staphylococcus aureus genetics
Staphylococcus aureus isolation & purification
Urinary Tract Infections microbiology
Cattle microbiology
Dairy Products microbiology
Intestines microbiology
Lactobacillus isolation & purification
Lactococcus lactis isolation & purification
Staphylococcus aureus pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 1432-072X
- Volume :
- 191
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Archives of microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 19529917
- Full Text :
- https://doi.org/10.1007/s00203-009-0492-9