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Interaction specificity of Arabidopsis 14-3-3 proteins with phototropin receptor kinases.
- Source :
-
FEBS letters [FEBS Lett] 2009 Jul 07; Vol. 583 (13), pp. 2187-93. Date of Electronic Publication: 2009 Jun 12. - Publication Year :
- 2009
-
Abstract
- Phototropin receptor kinases play an important role in optimising plant growth in response to blue light. Much is known regarding their photochemical reactivity, yet little progress has been made to identify downstream signalling components. Here, we isolated several interacting proteins for Arabidopsis phototropin 1 (phot1) by yeast two-hybrid screening. These include members of the NPH3/RPT2 (NRL) protein family, proteins associated with vesicle trafficking, and the 14-3-3 lambda (lambda) isoform from Arabidopsis. 14-3-3lambda and phot1 were found to colocalise and interact in vivo. Moreover, 14-3-3 binding to phot1 was limited to non-epsilon 14-3-3 isoforms and was dependent on key sites of receptor autophosphorylation. No 14-3-3 binding was detected for Arabidopsis phot2, suggesting that 14-3-3 proteins are specific to phot1 signalling.
- Subjects :
- 14-3-3 Proteins metabolism
Arabidopsis enzymology
Arabidopsis Proteins metabolism
Binding Sites
Phosphoproteins chemistry
Phosphorylation
Phylogeny
Protein Serine-Threonine Kinases
Sequence Alignment
Signal Transduction
Substrate Specificity
Two-Hybrid System Techniques
14-3-3 Proteins chemistry
Arabidopsis metabolism
Arabidopsis Proteins chemistry
Phosphoproteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3468
- Volume :
- 583
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 19524572
- Full Text :
- https://doi.org/10.1016/j.febslet.2009.06.011