Back to Search
Start Over
Fabrication and characterization of silk fibroin-derived curcumin nanoparticles for cancer therapy.
- Source :
-
International journal of nanomedicine [Int J Nanomedicine] 2009; Vol. 4, pp. 115-22. Date of Electronic Publication: 2009 Apr 20. - Publication Year :
- 2009
-
Abstract
- Biologically derived nanoparticles (<100 nm) were fabricated for local and sustained therapeutic curcumin delivery to cancer cells. Silk fibroin (SF) and chitosan (CS) polymers were blended noncovalently to encapsulate curcumin in various proportions of SF and CS (75:25, 50:50, and 25:75 SF:CS) or pure SF at two concentrations (0.1% w/v and 10% w/v) using the devised capillary-microdot technique. Curcumin-polymer conjugates were frozen, lyophilized, crystallized, suspended in phosphate-buffered saline for characterization, and tested for efficacy against breast cancer cells. All nanoparticle formulations except 0.1% w/v 50:50 SFCS were less than 100 nm in size as determined with the transmission electron microscopy. The entrapment and release of curcumin over eight days was highest for SF-derived nanoparticles as compared to all SFCS blends. The uptake and efficacy of SF-coated curcumin was significantly higher (p < 0.001) than SFCS-coated curcumin in both low and high Her2/neu expressing breast cancer cells. Interestingly, the uptake of curcumin was highest for the high Her2/neu expressing breast cancer cells when delivered with a 10% w/v SF coating as compared to other formulations. In conclusion, SF-derived curcumin nanoparticles show higher efficacy against breast cancer cells and have the potential to treat in vivo breast tumors by local, sustained, and long-term therapeutic delivery as a biodegradable system.
- Subjects :
- Antineoplastic Agents administration & dosage
Antineoplastic Agents chemistry
Antineoplastic Agents pharmacokinetics
Breast Neoplasms pathology
Cell Line, Tumor
Curcumin administration & dosage
Diffusion
Humans
Nanoparticles ultrastructure
Breast Neoplasms metabolism
Curcumin chemistry
Curcumin pharmacokinetics
Drug Carriers chemistry
Fibroins chemistry
Nanoparticles chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1178-2013
- Volume :
- 4
- Database :
- MEDLINE
- Journal :
- International journal of nanomedicine
- Publication Type :
- Academic Journal
- Accession number :
- 19516890
- Full Text :
- https://doi.org/10.2147/ijn.s5581