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Anti-angiogenic effects of pterogynidine alkaloid isolated from Alchornea glandulosa.
- Source :
-
BMC complementary and alternative medicine [BMC Complement Altern Med] 2009 May 22; Vol. 9, pp. 15. Date of Electronic Publication: 2009 May 22. - Publication Year :
- 2009
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Abstract
- Background: Angiogenesis, a complex multistep process that comprehends proliferation, migration and anastomosis of endothelial cells (EC), has a major role in the development of pathologic conditions such as inflammatory diseases, tumor growth and metastasis. Brazilian flora, the most diverse in the world, is an interesting spot to prospect for new chemical leads, being an important source of new anticancer drugs. Plant-derived alkaloids have traditionally been of interest due to their pronounced physiological activities. We investigated the anti-angiogenic potential of the naturally occurring guanidine alkaloid pterogynidine (Pt) isolated from the Brazilian plant Alchornea glandulosa. The purpose of this study was to examine which features of the angiogenic process could be disturbed by Pt.<br />Methods: Human umbilical vein endothelial cells (HUVEC) were incubated with 8 muM Pt and cell viability, proliferation, apoptosis, invasion and capillary-like structures formation were addressed. Nuclear factor kappaB (NFkappaB), a transcription factor implicated in these processes, was also evaluated in HUVEC incubated with Pt. Quantifications were expressed as mean +/- SD of five independent experiments and one-way analysis of variance (ANOVA) followed by the Dunnet test was used.<br />Results: A significant decrease in proliferation and invasion capacity and an effective increase in apoptosis as assessed by bromodeoxyuridine (BrdU), double-chamber and terminal transferase dUTP nick end labeling (TUNEL) assay, respectively, have been found. Pt also led to a drastic reduction in the number of capillary-like structures formation when HUVEC were cultured on growth factor reduced-Matrigel (GFR-Matrigel) coated plates. In addition, incubation of HUVEC with Pt resulted in reduced NFkappaB activity.<br />Conclusion: These findings emphasize the potential use of Pt against pathological situations where angiogenesis is stimulated as tumor development.
- Subjects :
- Alkaloids chemistry
Alkaloids isolation & purification
Analysis of Variance
Angiogenesis Inhibitors isolation & purification
Antineoplastic Agents, Phytogenic isolation & purification
Apoptosis drug effects
Cell Line
Guanidines chemistry
Guanidines isolation & purification
Humans
In Situ Nick-End Labeling
NF-kappa B antagonists & inhibitors
Neoplasm Invasiveness
Plant Extracts chemistry
Plant Extracts isolation & purification
Plant Leaves
Plants, Medicinal chemistry
Umbilical Veins
Alkaloids pharmacology
Angiogenesis Inhibitors pharmacology
Antineoplastic Agents, Phytogenic pharmacology
Cell Proliferation drug effects
Endothelial Cells drug effects
Euphorbiaceae chemistry
Guanidines pharmacology
Plant Extracts pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1472-6882
- Volume :
- 9
- Database :
- MEDLINE
- Journal :
- BMC complementary and alternative medicine
- Publication Type :
- Academic Journal
- Accession number :
- 19463163
- Full Text :
- https://doi.org/10.1186/1472-6882-9-15