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Saliva of laboratory-reared Lutzomyia longipalpis exacerbates Leishmania (Leishmania) amazonensis infection more potently than saliva of wild-caught Lutzomyia longipalpis.
- Source :
-
Parasitology international [Parasitol Int] 2009 Sep; Vol. 58 (3), pp. 220-6. Date of Electronic Publication: 2009 May 18. - Publication Year :
- 2009
-
Abstract
- In order to compare the saliva effect from wild-caught and lab-reared L. longipalpis on the development of experimental cutaneous leishmaniasis, C57BL/6 mice were inoculated subcutaneously into the hind footpads with promastigotes of L. (L.) amazonensis plus salivary gland lysate from wild-caught (SGL-W) and lab-colonized (SGL-C) vectors. Lesion sizes were significantly larger in the mice infected with both saliva compared to mice infected with parasites alone; moreover, the lesions caused by parasite+SGL-C were significantly larger than the lesions caused by parasite+SGL-W. Histopathological morphometric studies regarding the acute phase of infections showed lower numbers of polymorphonuclear cells, greater numbers of mononuclear cells and parasites in SGL-C infected mice compared to SGL-W infected mice. In the chronic phase of infection, the number of mononuclear cells was lower and the number of parasites was greater in SGL-C infected mice than SGL-W infected mice. In vitro studies showed increased infection index of macrophages infected with parasites plus saliva compared to infection with parasites alone, with no difference between the saliva infection indices. SDS-PAGE gel for SGL-C and SGL-W showed differences in the composition and quantity of protein bands, determined by densitometry. These results call attention to the experimental saliva model, which shows exacerbation of infection caused by sandfly saliva.
- Subjects :
- Animals
Animals, Laboratory
Animals, Wild
Female
Insect Vectors parasitology
Insect Vectors pathogenicity
Leishmania classification
Leishmania growth & development
Leishmaniasis, Cutaneous immunology
Leishmaniasis, Cutaneous parasitology
Macrophages immunology
Macrophages parasitology
Mice
Mice, Inbred C57BL
Psychodidae parasitology
Saliva parasitology
Salivary Glands immunology
Leishmania pathogenicity
Leishmaniasis, Cutaneous pathology
Psychodidae physiology
Saliva immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-0329
- Volume :
- 58
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Parasitology international
- Publication Type :
- Academic Journal
- Accession number :
- 19454323
- Full Text :
- https://doi.org/10.1016/j.parint.2009.05.005