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Platelet-rich plasma and platelet-rich fibrin in human cell culture.
- Source :
-
Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics [Oral Surg Oral Med Oral Pathol Oral Radiol Endod] 2009 Jul; Vol. 108 (1), pp. 48-55. Date of Electronic Publication: 2009 May 17. - Publication Year :
- 2009
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Abstract
- Objectives: The clinical use of platelet-rich plasma (PRP) for preprosthetic surgery has been a matter of controversy until now. Only recently, a new blood preparation has been developed which results in platelet-rich fibrin (PRF). The objective of the present investigation was to examine the growth factor release from PRP and PRF in vitro.<br />Study Design: Whole blood samples from healthy participants (n = 10) were drawn to generate PRP and PRF. Human osteoblasts (O), human fibroblasts (F), and human osteoblast-derived osteosarcoma cells (Saos-2) were used for the cell culture. Cells of each cell line were cultivated, and PRP- or PRF-preparations added for ten days. The drawn medium was pooled and the quantities of growth factors (platelet-derived growth factor isomers AB and BB, insulin-like growth factor I, and transforming growth factor (TGF) isomers beta1 and beta2) analyzed by enzyme-linked immunosorbent assay.<br />Results: In osteoblast and Saos-2 cultures, cytokine concentrations were significantly higher for PRP than for PRF (P < .05). In fibroblast cultures, results were the same with the exception of TGF-beta2 (P < .05).<br />Conclusions: This study demonstrates that PRP application in cell cultures leads to higher levels of growth factors than PRF application.
- Subjects :
- Adult
Becaplermin
Cell Line
Cell Line, Tumor
Culture Media
Fibroblasts physiology
Humans
Insulin-Like Growth Factor I analysis
Insulin-Like Growth Factor I metabolism
Intercellular Signaling Peptides and Proteins analysis
Materials Testing
Middle Aged
Osteoblasts physiology
Osteosarcoma pathology
Platelet-Derived Growth Factor analysis
Platelet-Derived Growth Factor metabolism
Proto-Oncogene Proteins c-sis
Transforming Growth Factor beta1 analysis
Transforming Growth Factor beta1 metabolism
Transforming Growth Factor beta2 analysis
Transforming Growth Factor beta2 metabolism
Blood Platelets physiology
Fibrin physiology
Intercellular Signaling Peptides and Proteins metabolism
Platelet-Rich Plasma physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1528-395X
- Volume :
- 108
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics
- Publication Type :
- Academic Journal
- Accession number :
- 19451011
- Full Text :
- https://doi.org/10.1016/j.tripleo.2009.02.007