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Microbial changes in periodontitis successfully treated by mechanical plaque removal and systemic amoxicillin and metronidazole.
- Source :
-
International journal of medical microbiology : IJMM [Int J Med Microbiol] 2009 Aug; Vol. 299 (6), pp. 427-38. Date of Electronic Publication: 2009 May 15. - Publication Year :
- 2009
-
Abstract
- Scaling and root planing in conjunction with systemic administration of antibiotics is used for treatment of aggressive periodontitis. The study investigated the changes of the subgingival microbiota in a homogeneous cohort of 12 female Caucasian patients. Plaque samples were obtained from 4 defined deep lesions per patient at baseline and 2, 6, and 12 months after therapy (mechanical plaque removal, oral administration of amoxicillin and metronidazole). Amplification of the 16S rRNA gene, cloning, and sequencing were applied to identify microbial species. Porphyromonas gingivalis strains were typed by multilocus sequence typing. Despite of a favorable clinical outcome, 16S rRNA sequence analysis revealed only minor changes of the microbiota with a temporal reduction of P. gingivalis and of Treponema denticola-like phylotypes. In contrast to T. denticola, T. sokranskii-like phylotypes were not affected. In 4 patients with recurrent colonization by P. gingivalis, the bacterial clones were identical before and after therapy as evidenced by multilocus sequence typing suggesting clonal persistence or reinfection during the course of the study. In summary, despite a favorable clinical outcome, a transient effect on only few bacterial species was observed.
- Subjects :
- Adult
Bacteria classification
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
Female
Gingiva microbiology
Humans
Middle Aged
Periodontitis microbiology
Periodontitis therapy
Phylogeny
Porphyromonas gingivalis classification
Porphyromonas gingivalis isolation & purification
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Treponema classification
Treponema isolation & purification
Amoxicillin therapeutic use
Anti-Bacterial Agents therapeutic use
Bacteria isolation & purification
Biodiversity
Dental Scaling
Metronidazole therapeutic use
Periodontitis drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1618-0607
- Volume :
- 299
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- International journal of medical microbiology : IJMM
- Publication Type :
- Academic Journal
- Accession number :
- 19447073
- Full Text :
- https://doi.org/10.1016/j.ijmm.2009.03.001