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Oleoyl-chitosan nanoparticles inhibits Escherichia coli and Staphylococcus aureus by damaging the cell membrane and putative binding to extracellular or intracellular targets.
- Source :
-
International journal of food microbiology [Int J Food Microbiol] 2009 Jun 30; Vol. 132 (2-3), pp. 127-33. Date of Electronic Publication: 2009 Apr 22. - Publication Year :
- 2009
-
Abstract
- A novel chitosan antibacterial dispersion system was prepared by oleoyl-chitosan (OCS) nanoparticles (OCNP). We further investigated the antimicrobial mode of OCNP against Escherichia coli and Staphylococcus aureus using a combination of approaches, including measurement of the effect of lecithin and phosphate groups, the conformation of membrane protein, internalization of fluorescein isothiocyanate (FITC)-labeled OCS nanoparticles (FITC-OCS nanoparticles) observed under fluorescence microscopy and DNA/RNA binding assay. Results of fluorescence experiments indicated that OCNP influenced the structure of bacterial membranes. The lecithin effect showed that OCNP bound to cytoplasmic membrane phospholipids of S. aureus, and phosphate groups played an important role. Fluorescence microscopy observations demonstrated that the way OCNP entered into bacteria varied against strains. The gel-retardation experiment showed that OCNP bound strongly to DNA/RNA and retarded their migration in the gels in a concentration-dependent manner. These results indicate that OCNP exerts its antibacterial activity by damaging the structures of cell membrane and putative binding to extracellular targets such as phosphate groups or intracellular targets such as DNA and RNA.
- Subjects :
- Dose-Response Relationship, Drug
Escherichia coli growth & development
Fluorescein-5-isothiocyanate
Gels
Microbial Sensitivity Tests
Microscopy, Fluorescence
Nanoparticles
Oleic Acid
Staphylococcus aureus growth & development
Anti-Bacterial Agents pharmacology
Cell Membrane drug effects
Chitosan pharmacology
Escherichia coli drug effects
Staphylococcus aureus drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3460
- Volume :
- 132
- Issue :
- 2-3
- Database :
- MEDLINE
- Journal :
- International journal of food microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 19439383
- Full Text :
- https://doi.org/10.1016/j.ijfoodmicro.2009.04.013